In Situ Hybridization Protocols

Inbunden, Engelska, 2014

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In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules.

Produktinformation

Utgivningsdatum2014-09-13
Mått178 x 254 x 17 mm
Vikt676 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor275
Upplaga4
FörlagHumana Press Inc.
ISBN9781493914586

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Medicinsk genetik inom Medicin

Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae.- MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos.- Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies.- Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian.- In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae.- LNA-based in Situ Hybridization Detection of mRNAs in Embryos.- Chromogen Detection of microRNA in Frozen Clinical Tissue Samples using LNA™ Probe Technology.- MicroRNA In Situ Hybridisation in Tissue Microarrays.- Fluorescence in situ Hybridization for Detection of Small RNAs on Frozen Tissue Sections.- Sensitive and Specific in situ Hybridisation for Early Drug Discovery.- Zinc Based Fixation for High Sensitivity in situ Hybridization: A Non-Radioactive Colorimetric Method for the Detection of Rare Transcripts on Tissue Sections.- Dual-color Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope.- Fully Automated Fluorescence-based 4-color Multiplex Assay for Co-detection of microRNA and Protein Biomarkers in Clinical Tissue Specimens.- Multiplexed miRNA Fluorescence in situ Hybridization for Formalin-Fixed Paraffin-Embedded Tissues.- Simultaneous Detection of Nuclear and Cytoplasmic RNA Variants Utilizing Stellaris® RNA Fluorescence in situ Hybridization in Adherent Cells.- Quantitative Ultrasensitive Bright-field RNA in situ Hybridization with RNAscope.- Identification of Low Expressing Transcripts of the NPY Receptors’ Family in the Murine Lingual Epithelia.- In Situ Hybridization for Fungal Ribosomal RNA Sequences in Paraffin-Embedded Tissues Using Biotin-Labeled Locked Nucleic Acid Probes.- In situ Hybridization Freeze-assisted Punches (IFAP): Technique for Liquid-based TissueExtraction from Thin Slide-mounted Sections for DNA Methylation Analysis.- miRNA Detection at Single Cell Resolution Using Microfluidic LNA flow-FISH.- PNA-based Fluorescence in situ Hybridization for Identification of Bacteria in Clinical Samples.