Handbook of ELISPOT

Methods and Protocols

Häftad, Engelska, 2010

1 369 kr

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In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

Produktinformation

Utgivningsdatum2010-11-19
Mått152 x 229 x 19 mm
Vikt499 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor322
FörlagHumana Press Inc.
ISBN9781617375880

Tillhör följande kategorier

Onkologi inom Medicin

Elispot Assay Setup and Data Analysis.- ELISPOT Assay.- Chemistry and Biology of the ELISPOT Assay.- Membranes and Membrane Plates Used in ELISPOT.- Standardization and Validation Issues of the ELISPOT Assay.- A Cell-Detachment Solution Can Reduce Background Staining in the ELISPOT Assay.- Isolation of Subsets of Immune Cells.- Image Analysis and Data Management of ELISPOT Assay Results.- High Resolution as a Key Feature to Perform Accurate ELISPOT Measurements Using Zeiss KS ELISPOT Readers.- Elispot Assay in Animal Models.- Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques.- Feline Cytokine ELISPOT.- Measuring T-Cell Function in Animal Models of Tuberculosis by ELISPOT.- Interferon-? ELISPOT Assay for the Quantitative Measurement of Antigen-Specific Murine CD8+ T-Cells.- Elispot Assays in Human Studies.- Detection of Measles Virus-Specific Interferon-?-Secreting T-Cells by ELISPOT.- Epitope Mapping in Multiple Sclerosis Using the ELISPOT Assay.- Use of Interferon-? ELISPOT in Monitoring Immune Responses in Humans.- ELISPOT Determination of Interferon-? T-Cell Frequencies in Patients With Autoimmune Sensorineural Hearing Loss.- Multiplex and Modified Elispot Assay Formats.- Dual-Color ELISPOT Assay for Analyzing Cytokine Balance.- Simultaneous Detection of Multiple Cytokines in ELISPOT Assays.- Fluorospot Assay.- A Gel-Based Dual Antibody Capture and Detection Method for Assaying of Extracellular Cytokine Secretion.

From the reviews: "The book is divided into four sections, with a total of 20 chapters featuring step-by-step protocols that will enable both novice and experienced researchers to perform ELISPOT. Each chapter begins with a general Introduction, followed by a detailed Materials section and an easy-to-follow Methods section ... . All the chapters are equally interesting and well presented ... . a must for a cytochemist. This book will serve as a convenient reference manual for beginners and for experienced ELISPOT users." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)