Environmental DNA

For Biodiversity Research and Monitoring

Häftad, Engelska, 2018

Av Pierre Taberlet, Aurélie Bonin, Lucie Zinger, Eric Coissac, Aurelie Bonin, France) Taberlet, Pierre (Senior CNRS scientist, Senior CNRS scientist, Universite Grenoble Alpes, France) Bonin, Aurelie (Research scientist, Research scientist, Universite Grenoble Alpes, France) Zinger, Lucie (Assistant professor, Assistant professor, Ecole Normale Superieure de Paris, France) Coissac, Eric (Associate professor, Associate professor, Universite Grenoble Alpes

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Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.

Produktinformation

Utgivningsdatum2018-02-15
Mått190 x 248 x 13 mm
Vikt576 g
FormatHäftad
SpråkEngelska
Antal sidor272
FörlagOUP OXFORD
ISBN9780198767282

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After teaching biology in high school for 10 years, Pierre Taberlet obtained his PhD in ecology in 1992 at the Université Grenoble Alpes. He is currently senior CNRS scientist in Laboratoire d'Ecologie Alpine at the Université Grenoble Alpes. He has been heavily involved in developing innovative molecular tools for tackling ecological questions. He was the recipient of the Molecular Ecology Prize in 2007 and is a member of the Norwegian Academy of Sciences and Letters (2005) and of Academia Europaea (2014).Aurélie Bonin obtained her PhD in genomics of local adaption in 2006. After various postdoctoral positions in population genomics and ecology, she is now a DNA metabarcoding project leader for the French AnaEE (Analysis and Experimentation on Ecosystems).Lucie Zinger obtained her PhD in microbial ecology in 2009. She is currently assistant professor at the Institut de Biologie de l'École Normale Supérieure in Paris. She is interested in the fields of biogeography, macroecology, community ecology, and microbial ecology, as well as eDNA-based techniques to uncover the diversity of elusive organisms. Her current work focuses on the distribution and assembly mechanisms of microorganisms in soils, oceans, plant leaves, and insects.Geneticist and bioinformatician, Eric Coissac obtained his PhD in genetics and genomics in 1996 at the Université Pierre et Marie Curie in Paris. He is currently associate professor at Université Grenoble Alpes in Laboratoire d'Écologie Alpine.

1: Introduction to environmental DNA (eDNA)2: DNA metabarcode choice and design3: Reference databases4: Sampling5: DNA extraction6: DNA amplification and multiplexing7: DNA sequencing8: DNA metabarcoding data analysis9: Single-species detection10: Environmental DNA for functional diversity11: Some early landmark studies12: Freshwater ecosystems13: Marine environments14: Terrestrial ecosystems15: Palaeoenvironments16: Host-associated microbiota17: Diet analysis18: Analysis of bulk samples19: The future of eDNA metabarcoding