Electron Microscopy of Model Systems

Inbunden, Engelska, 2010

2 509 kr

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The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of�the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems.

Covers the preparation and analysis of model systems for biological electron microscopy
Includes the most popular systems but also organisms that are less frequently used in cell biology
Presents the currently most important methods for the preparation of biological specimens
First compendium covering the various aspects of sample preparation of very diverse biological systems

Produktinformation

Utgivningsdatum2010-09-24
Mått191 x 235 x undefined mm
Vikt1 400 g
FormatInbunden
SpråkEngelska
SerieMethods in Cell Biology
Antal sidor744
FörlagElsevier Science
MedarbetareThomas Muller-Reichert
ISBN9780123810076

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Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy.

Preface/AcknowledgementsThomas Müller-Reichert1. Electron microscopy of virusesMichael Laue2. Bacterial TEM: New insights from cryo-microscopyMartin Pilhofer, Mark S. Ladinsky, Alasdair W. McDowall, and Grant Jensen* 3. Analysis of the ultrastructure of archaea by electron microscopyReinhard Rachel* , Carolin Meyer, Andreas Klingl, Sonja Gürster, Nadine Wasserburger, Ulf Küper, Annett Bellack, Simone Schopf, Reinhard Wirth, Harald Huber, and Gerhard Wanner4. Chlamydomonas: Cryopreparation methods for the 3-D analysis of cellular organellesEileen T. O’Toole 5. Ultrastructure of the asexual blood stages of Plasmodium falciparumEric Hanssen* , Kenneth N Goldie, and Leann Tilley6. Electron tomography and immunolabeling of Tetrahymena thermophila basal bodies Thomas H. Giddings* Jr., Janet B. Meehl, Chad G. Pearson, and Mark Winey7. Electron microscopy of ParameciumKlaus Hausmann* and Richard D. Allen8. Ultrastructural investigation methods for Trypanosoma bruceiJohanna L. Höög* , Eva Gluenz, Sue Vaughan, and Keith Gull9. Dictyostelium discoideum: A model system for ultrastructural analyses of cell motility and developmentMichael P. Koonce* and Ralpf Gräf10. Towards sub-second correlative live-cell light and electron microscopy of Saccharomyces cerevisiaeChristopher Buser11. Fission yeast: A cellular model well suited for electron microscopic investigationsHélio D. Roque and Claude Antony* 12. High-pressure freezing and electron microscopy of ArabidopsisByung-Ho Kang13. Standard and cryo-preparation of Hydra for transmission electron microscopyThomas W. Holstein* , Michael W. Hess, and Willi Salvenmoser14. Electron microscopy of flatworms: Standard and cryopreparation methodsWilli Salvenmoser* , Bernhard Egger, Johannes G. Achatz, Peter Ladurner, and Michael W. Hess* 15. Three-dimensional reconstruction methods for C. elegans ultrastructureThomas Müller-Reichert* , Joel Mancuso, Ben Lich, and Kent McDonald* 16. Insects as model systems in cell biologyThomas A. Keil* and R. Alexander Steinbrecht17. Electron microscopy of amphibian model systems (Xenopus laevis and Ambystoma mexicanum)Thomas Kurth* , Jürgen Berger, Michaela Wilsch-Bräuninger, Robert Cerny, Heinz Schwarz, Jan Löfberg, Thomas Piendl, and Hans Epperlein18. Modern approaches for ultrastructural analysis of the zebrafish embryoNicole L. Schieber, Susan J. Nixon, Richard I. Webb* , and Robert G. Parton* 19. Transmission electron microscopy of cartilage and boneDouglas R. Keene* , and Sara F. Tufa20. Electron microscopy of the mouse central nervous systemWiebke Möbius* , Frédérique Varoqueaux, Benjamin H. Cooper, Cordelia Imig, Torben Ruhwedel, Aiman S. Saab, and Walter Kaufmann21. Rapidly excised and cryofixed rat tissueDimitri Vanhecke, Werner Graber, and Daniel Studer* 22. The actin cytoskeleton in whole mounts and sectionsGuenter P. Resch* , Edit Urban, and Sonja Jacob23. Three-dimensional cryo-electron microscopy on intermediate filaments: A critical comparison to other structural methodsRobert Kirmse, Cédric Bouchet-Marquis, Cynthia Page, and Andreas Hoenger* 24. Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cellsJulien Guizetti, Jana Mäntler, Thomas Müller-Reichert* , and Daniel W. Gerlich* 25. Viral infection of cells in culture: Approaches for electron microscopyPaul Walther* , Li Wang, Sandra Ließem, and Giada Frascaroli26. Intracellular membrane traffic at high resolutionJan R. T. van Weering, Edward Brown, Thomas H. Sharp, Judith Mantell, Peter J. Cullen, and Paul Verkade* 27. 3D versus 2D cell culture: Implications for electron microscopyMichael W. Hess* , Kristian Pfaller, Hannes L. Ebner, Beate Beer, Daniel Hekl, and Thomas Seppi28. ‘Tips and tricks’ for high-pressure freezing of model systemsKent McDonald* , Heinz Schwarz, Thomas Müller-Reichert, Rick Webb, Christopher Buser, and Mary Morphew