Cytochrome P450 Protocols

• Bioluminescent Assays for Cytochrome P450 Enzymes. In Vitro Drug-Drug Interaction Assay.- High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening.- The Synthesis, Characterization and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites.- High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators.- Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches.- Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium Tuberculosis.- An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins.- Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group.- Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes.- Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles.- Fluorescence-Based Screening of cytochrome P450 activities in Intact Cells.- Screening for Cytochrome P450 Reactivity with a Reporter Enzyme.- High-Throughput Fluorescence Assay of Cytochrome P450 3A4.- Targeted Protein Capture for Analysis of Electrophile-Protein Adducts.- DNA Shuffling of Cytochrome P450 Enzymes.- Measurement of P450 Difference Spectra Using Intact Cells.- DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production.- P450 oxidoreductase – Genotyping, Expression, Purification of Recombinant Protein and Activity Assessments of Wild-Type and Mutant Protein.- LICRED: A Versatile Drop-In Vector for Rapid Generation of Redox-Self-Sufficient Cytochromes P450.- Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-allele) Nomenclature Database.- Simultaneous in vivo Phenotyping of CYP Enzymes.- Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase-I Footprinting.- Isolation of MouseHepatocytes.- Highly Efficient SiRNA and Gene Transfer into Hepatocyte-like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies.

From the reviews of the third edition:“With this third edition, the authors and contributors continue to provide what has become a tour de force of bench top manuals for the cytochrome P450 field. … this book should also prove of interest to graduate students and postdoctoral researchers who are new to the field of P450 science, or who would like to expand their research directions. … This stands alone as the premier book for this field, which is sorely lacking in publications of this type.” (Jed N. Lampe, Doody’s Book Reviews, October, 2013)