Vectorology for Optogenetics and Chemogenetics

Inbunden, Engelska, 2023

AvMark A.G. Eldridge,Adriana Galvan,Mark A. G. Eldridge

3 119 kr

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The chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression.

Produktinformation

Utgivningsdatum2023-02-07
Mått178 x 254 x 25 mm
Vikt852 g
FormatInbunden
SpråkEngelska
SerieNeuromethods
Antal sidor333
FörlagSpringer-Verlag New York Inc.
ISBN9781071629178

Tillhör följande kategorier

Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain.- HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport.-Generation of High-Titer Defective HSV-1 Amplicon Vectors.- Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research.- Quality control for Adeno-associated viral vector production.- Vector tropism.-Viruses for systemic delivery.- Transcriptomic Definition of Neuron Types.- Enhancers for selective targeting.- Pathway-selective reversible perturbations using a double-infection technique in the macaque brain.- Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs.- Convection-enhanced delivery of viral vectors.- Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain.- Methods to verify expression and function of DREADDs using PET.- Reporter selection and post-mortem methods to verify transgene expression.- Considerations for the use of viral vectors in NHP experiments.