Ultrastructural Imaging of COVID-19

Subhash Chandra Yadav is an assistant professor of electron microscopy and nanotechnology at the All India Institute of Medical Sciences (AIIMS), New Delhi, India, since June 2015 and is currently working as an additional professor. He earned his PhD from the Institute of Medical Sciences, Banaras Hindu University (BHU), Varanasi, India. He has been a visiting researcher at Free University (FU) Berlin, Germany, and a postdoctoral fellow at the Indian Institute of Science (IISc), Bengaluru, India, and the University of Tennessee Medical Center, Knoxville, TN, USA. He has worked as a scientist in charge of the electron microscopy facility at the Institute of Himalayan Bioresource Technology (CSIR-IHBT) in Palampur, India, and the TERI-Deakin University Nanobiotechnology Centre. He has also been a visiting professor at Stanford University, USA. Dr Yadav has significantly utilized electron microscopy for the last 18 years to develop cost-effective and visual nanodiagnostic devices for prostate and cervical cancer and the BK virus, various nanoformulations for value addition, and targeted delivery vehicles. He worked extensively during the COVID-19 pandemic to explore the ultrastructural impact of SARS-CoV-2 infection in patients with acute respiratory distress syndrome (ARDS), including young and old patients with and without comorbidities, such as diabetes, liver and kidney disease, and malignancy.

• 1. SARS-CoV-2 and COVID-19: An Ultrastructural Perspective 2. Ultrastructural and Molecular Architecture of SARS-CoV-2 3. Tropism of SARS-CoV-2: Microscopic Analysis 4. Impact of SARS-CoV-2 in the Ultrastructure of Keratinized Oral Epithelium 5. Impact of SARS-CoV-2 in the Ultrastructure of Non-Keratinized Oral Epithelium 6. Ultrastructure of Different Cells of the Nasal Cavity of COVID-19 Patients 7. Ultrastructural Alteration of Ciliated Epithelium of Bronchoalveolar Lavage Fluids 8. Ultrastructural Variation of Type II Pneumocytes of BALF of COVID-19 Patients 9. Ultrastructural Modification of Alveolar Macrophage of BALF 10. Ultrastructure of Lung-Infiltrated Neutrophils 11. Ultrastructural Modification of Eosinophils in BALF of SARS-CoV-2 Patients 12. Ultrastructural Proof of SARS-CoV-2 Infection in Lymphocytes 13. Ultrastructural Modification of Anucleated Granulocytes in BALF 14. SARS-CoV-2-Induced Formation of Single and Heterotypic Syncytial Cells: An Ultrastructural Confirmation 15. Ultrastructural Evidence for the Impact of Prophylactic Hydroxychloroquine on Impairment and Cellular SARS-CoV-2 Infection in Different Cells of BALF of COVID-19 Patients 16. SARS-CoV-2-Induced Ultrastructural Changes in the Ciliated Epithelium, Type II Pneumocytes, Macrophages, and Neutrophils of COVID-19 Patients 17. Abnormal Ultrastructure Features on Type II Pneumocytes of COVID-19 Patients with Aplastic Anemia