Del 423 - Methods in Enzymology

Two-Component Signaling Systems, Part B

Inbunden, Engelska, 2007

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Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections in Two-Component Signaling Systems, Part B include:

Structural Approaches
Reconstitution of Heterogeneous Systems
Intracellular Methods and Assays
Genome-Wide Analyses of Two-Component Systems

Presents detailed protocols
Includes troubleshooting tips

Produktinformation

Utgivningsdatum2007-07-26
Mått152 x 229 x undefined mm
Vikt1 330 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor648
FörlagElsevier Science
ISBN9780123738523

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Biokemi inom Naturvetenskap och teknik

Section I: Structural Approaches[1]: The PICM Chemical Scanning Method for Identifying Domain–Domain and Protein–Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis[2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis[3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases[4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR[5]: TonB/TolA Amino-Terminal Domain Modeling[6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques[7]: The Design and Development of Tar-EnvZ Chimeric Receptors[8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli[9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis[10]: A Temperature-Sensing Histidine Kinase—Function, Genetics, and Membrane Topology[11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal MoleculesSection II: Reconstitution of Heterogeneous Systems[12]: Liposome-Mediated Assembly of Receptor Signaling Complexes[13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines[14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers[15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases[16]: Genetic Dissection of Signaling Through the Rcs PhosphorelaySection III: Intracellular Methods and Assays[17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis[18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes[19]: In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays[20]: A “Bucket of Light for Viewing Bacterial Colonies in Soft Agar[21]: Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors[22]: Single-Cell Analysis of Gene Expression by Fluorescence MicroscopySection IV: Genome-Wide Analyses of Two-Component Systems[23]: Two-Component Systems of Mycobacterium tuberculosis—Structure-Based Approaches[24]: Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus[25]: Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling[26]: Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays[27]: Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based TechniquesSubject IndexAuthor Index