Tissue Microarrays

Methods and Protocols

Häftad, Engelska, 2016

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The tissue microarray (TMA) method presents as a modern high technology, although its roots go back to the 80s when researchers frst started to combine several small pieces of tissues into so-called sausage blocks.

Produktinformation

Utgivningsdatum2016-08-23
Mått178 x 254 x 12 mm
Vikt395 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor193
FörlagHumana Press Inc.
ISBN9781493961030

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik

Applications of Tissue Microarray Technology.- Quality Aspects of TMA Analysis.- Representativity of TMA Studies.- Recipient Block TMA Technique.- Protocol for Constructing Tissue Arrays by Cutting Edge Matrix Assembly.- Hypodermic Needle Without Recipient Paraffin Block Technique.- Resin Technologies: Construction and Staining of Resin TMA’s.- Tissue Microarrays from Frozen Tissues-OCT Technique.- An Alternative Technology to Prepare Tissue Microarray Using Frozen Tissue Samples.- Building “Tissue” Microarrays from Suspension Cells.- Tissue Microarrays from Biopsy Specimens.- Immunohistochemical Analysis of Tissue Microarrays.- DNA Copy Number Analysis on Tissue Microarrays.- RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded Tissues in TMA Format by RNA In Situ Hybridization.- Automated Analysis of Tissue Microarrays.- Digital Microscopy for Boosting Database Integration and Analysis in TMA Studies.- From Gene to Clinic: TMA-Based Clinical Validation of Molecular Markers in Prostate Cancer.

From the reviews:“This book is the first … to attempt to summarise the field of Tissue Microarrays (TMAs) since the first report of the technology in 1998. … relevant to the clinician-scientist who may be considering high-throughput analyses of genes and proteins from a large cohort. … In summary, this book is crammed with incredibly detailed technical advice … and even advice on whether to drill or cut the recipient paraffin blocks. … very readable, very informative and an excellent introduction to the gentle art of TMAs.” (Cris dos Remedios and Sean Lal, Proteomics, Vol. 11, 2011)