The Immunological Synapse Part A

Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

• Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: Control by polarity regulatorsMarie Juzans, Celine Cuche, Vincenzo Di Bartolo, and Andres AlcoverAnalysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapseSara Fernandez-Hermira, Irene Sanz-Fernandez, Marta Botas, Victor Calvo, and Manuel IzquierdoP815-based redirected degranulation assay to study human NK cell effector functionsIñigo Terren, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego Cytotoxic and chemotactic dynamics of NK cells quantified by live-cell imagingYanting Zhu and Jue ShiQuantification of interaction frequency between antigen-presenting cells and T cells by conjugation assayOndrej CernyAssessment of membrane lipid state at the natural killer cell immunological synapseYu Li and Jordan S. OrangeStudy of the effects of NK-tumor cell interaction by proteomic analysis and imagingChiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica ParodiProtocol for the murine antibody-dependent cellular phagocytosis assayEliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia VascottoQuantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titersMelanie Grusdat, Catherine Dostert, and Dirk BrennerAn in vitro model to monitor natural killer cell effector functions against breast cancer cells derived from human tumor tissueNicky A. Beelen, Femke A.I. Ehlers, Loes F.S. Kooreman, Gerard M.J. Bos, and Lotte WietenStandardized protocol for the evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayerJong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang LiuPotency monitoring of CAR T cellsDongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine E. Brown