The Chlamydomonas Sourcebook

Volume 2: Organellar and Metabolic Processes

Inbunden, Engelska, 2023

Av Arthur Grossman, Francis-Andr� Wollman, Francis-André Wollman, Stanford University.) Grossman, Arthur (Staff scientist, Carnegie Institution for Science<br>Courtesy professor, Centre National de la Recherche Scientifique (CNRS).) Wollman, Francis-Andre (Emeritus research director

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Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism.

This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes.

Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism
Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies
Assists researchers with common laboratory problems such as contamination

Produktinformation

Utgivningsdatum2023-02-22
Mått216 x 279 x 46 mm
Vikt2 270 g
FormatInbunden
SpråkEngelska
Antal sidor864
Upplaga3
FörlagElsevier Science
ISBN9780128214305

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Arthur R. Grossman has been a staff scientist at the Carnegie Institution for Science since 1982 and is a courtesy professor at Stanford University. He received both the Gilbert Morgan Smith Medal (2009) from the National Academy of Sciences and the Darbaker Prize (2002) from the Botanical Society of America for his work on microalga. In an NSF-supported project and collaborations with the Joint Genome Institute of the Department of Energy (DOE), he spearheaded the initial Chlamydomonas genome project that led to the complete Chlamydomonas genome sequence, its initial annotation, and the use of the information to promote genome-wide transcriptome analyses; Chlamydomonas remains a powerful molecular-genomic model system and a flagship alga of the DOE. Grossman’s focus is on how photosynthetic organisms perceive and respond to their environment, with an emphasis on light and nutrient conditions. Francis-André Wollman is an emeritus research director at the Centre National de la Recherche Scientifique (CNRS), a member of the French Academy of Sciences, a member of EMBO, and has received the Silver Medal from the CNRS. In the mid-1970s, he joined the photosynthesis laboratory of Pierre Joliot at the Institut de Biologie Physico-Chimique (IBPC) in Paris and in the late 1990s, he became the director of this laboratory before being appointed director of IBPC in 2007. His research has focused on the biogenesis, regulation, and evolution of oxygenic photosynthesis. Throughout his career, he has used the genetics of Chlamydomonas reinhardtii for biophysical, biochemical, and structural studies to provide a dynamic view of photosynthesis as being highly responsive to an ever-changing environment through its bioenergetic integration and metabolic flexibility.

1. The Comparative, Biochemistry, Genetics and Evolution of Starch Metabolism in Chlamydomonas reinhardtii2. Chlamydomonas Glycerolipid Metabolism3. Nitrogen Metabolism in Chlamydomonas4. Phosphorus and Sulfur Uptake, Assimilation, and Deprivation Responses5. Trace metal nutrition and response to deficiency 6. Sensory photoreceptors in Chlamydomonas7. RubisCo and Carbon assimilation8. Photoproduction of reducing power and the Calvin-Benson Cycle9. Metabolic Networks during Dark Anoxia10. Hydrogenases and hydrogen production11. The mitochondrion: from genome to proteome 12. The chloroplast in a changing environment: from genome to proteome 13. Control of organellar gene expression by nucleus-encoded proteins14. Translation and Protein Synthesis in the chloroplast15. Photosynthesis: Light Harvesting16. Photosystem I and II17. Chloroplast ATP synthase and the cytochrome b6f complex18. The multiple routes of photosynthetic electron transfer in Chlamydomonas reinhardtii19. Assembly of Photosynthetic proteins20. Molecular chaperones and proteases21. Tetrapyrrole biosynthesis and signaling (chlorophyll heme and bilins)22. Carotenoids in Chlamydomonas23. Supramolecular organization of chloroplast membranes24. State transitions25. Photoprotection