TET Proteins and DNA Demethylation

Methods and Protocols

Häftad, Engelska, 2021

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Part Four discusses protocols that detail how to purify TET proteins and unravel their protein interactions, and Part Five looks at the assessment of TET protein function and activity in vivo and in vitro.

Produktinformation

Utgivningsdatum2021-05-20
Mått178 x 254 x 19 mm
Vikt626 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor320
FörlagSpringer-Verlag New York Inc.
ISBN9781071612965

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Medicinsk genetik inom Medicin
Biokemi inom Naturvetenskap och teknik

Reduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine.- Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine.- Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome Bisulfite Sequencing (WGBS).- ELISA-Based Quantitation of Global 5hmC Levels.- Avidin-Biotin ELISA-Based Detection of 5hmC.- Quantification of DNA Methylation and Its Oxidized Derivatives using LC-MS.- Distinguishing Active Versus Passive DNA Demethylation using Illumina MethylationEPIC BeadChip Microarrays.- Bioinformatic Estimation of DNA Methylation and Hydroxymethylation Proportions.- TAB-seq and ACE-seq Data Processing for Genome-Wide DNA Hydroxymethylation Profiling.- Genomic Targeting of TET Activity for Targeted Demethylation using CRISPR/Cas9.- High-Fidelity CRISPR/Cas9-Based Gene-Specific Hydroxymethylation.- Identifying Protein–(hydroxy)methylated DNA Interactions using Quantitative Interaction Proteomics.- Purification of TET Proteins.- Uncovering Sequence Specific Transcription Factors Interacting with TET2.- ChIP-Sequencing of TET Proteins.- Harnessing Alternative Substrates to Probe TET Family Enzymes.- Generation and Molecular Characterization of Transient tet1/2/3 Zebrafish Knockouts.