Targeting Enzymes for Pharmaceutical Development

Methods and Protocols

Häftad, Engelska, 2020

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This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals.

Produktinformation

Utgivningsdatum2020-11-27
Mått178 x 254 x 17 mm
Vikt576 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor290
FörlagSpringer-Verlag New York Inc.
ISBN9781071601655

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In Silico Laboratory: Tools for Similarity-Based Drug Discovery.- The In Silico Fischer Lock-and-Key Model: The Combined Use of Molecular Descriptors and Docking Poses for the Repurposing of Old Drugs.- Determination of Half-Maximal Inhibitory Concentration of an Enzyme Inhibitor.- Applications of Differential Scanning Fluorometry and Related Technologies in Characterization of Protein-Ligand Interactions.- High-Throughput Differential Scanning Fluorimetry of GFP-Tagged Proteins.- Enzyme-Ligand Interaction Monitored by Synchrotron Radiation Circular Dichroism.- Measuring Small Molecule Binding to Escherichia coli AcrB by Surface Plasmon Resonance.- Systematic Screening of Viral Entry Inhibitors Using Surface Plasmon Resonance.- Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry.- Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA).- On-Line Enantioselective Capillary Electrophoretic Method for Screening Cytochrome P450 3A4Inhibitors.- Enzymatic Bioautographic Methods.- High-Throughput Assessment of Metabolism-Induced Toxicity of Compounds on a 384-Pillar Plate.- Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors.- Ligand Fishing: An Approach for the Discovery Inhibitors from Complex Biological Mixtures.- HMG-CoA Reductase as Target for Drug Development.- Lipoxygenases as Targets for Drug Development.- Cholinesterase as a Target for Drug Development in Alzheimer’s Disease.