Synthetic Biology and Metabolic Engineering in Plants and Microbes Part B: Metabolism in Plants

John Innes Centre, Norwich Research Park, UK

• 1. Comparative Biochemistry and in Vitro Pathway Reconstruction as Powerful Partners in Studies of Metabolic DiversityPengxiang Fan, Gaurav D. Moghe and Robert L. Last2. De Novo Deep Transcriptome Analysis of Medicinal Plants for Gene Discovery in Biosynthesis of Plant Natural ProductsRongchun Han, Amit Rai, Michimi Nakamura, Hideyuki Suzuki, Hiroki Takahashi, Mami Yamazaki and Kazuki Saito3. Genomics Based Discovery of Plant Genes for Synthetic Biology of Terpenoid Fragrances: A Case Study in Sandalwood Oil BiosynthesisJose M. Celedon  and Jörg Bohlmann4. A Workflow for Studying Specialized Metabolism in Non-Model Eukaryotic OrganismsMichael P. Torrens-Spence, Timothy R. Fallon and  Jing-Ke Weng5. Gene Discovery for Synthetic Biology: Exploring the Novel Natural Product Biosynthetic Capacity of Eukaryotic MicroalgaeEllis C. O’Neill, Gerhard Saalbach and Robert A. Field6. Cis-Prenyltransferase and Polymer Analysis from a Natural Rubber PerspectiveMoonhyuk Kwon, Eun-Joo Gina Kwon and Dae-Kyun Ro7. Generation and Functional Evaluation of Designer Monoterpene Synthases Narayanan Srividya, Iris Lange and Bernd Markus Lange8. Prequels to Synthetic Biology: From Candidate Gene Identification and Validation to Enzyme Subcellular Localization in Plant and Yeast CellsEmilien Foureau, Inês Carqueijeiro, Thomas Dugé de Bernonville, Céline Melin, Florent Lafontaine, Sébastien Besseau, Arnaud Lanoue, Nicolas Papon, Audrey Oudin, Gaëlle Glévarec, Marc Clastre, Benoit St-Pierre, Nathalie Giglioli-Guivarc’h and Vincent Courdavault9. Functional Expression and Characterization of Plant ABC Transporters in Xenopus Laevis Oocytes for Transport Engineering PurposesDeyang Xu, Dorottya Veres, Zeinu Mussa Belew, Carl Erik Olsen, Hussam Hassan Nour-Eldin and Barbara Ann Halkier10. Quantifying the Metabolites of the Methylerythritol 4-Phosphate (Mep) Pathway in Plants and Bacteria by Liquid Chromatography – Triple Quadrupole Mass SpectrometryDiego González-Cabanelas, Almuth Hammerbacher, Bettina Raguschke, Jonathan Gershenzon and Louwrance P. Wright11. Establishing the Architecture of Plant Gene Regulatory NetworksFan Yang, Wilberforce Zachary Ouma, Wei Li, Andrea I. Doseff, and Erich Grotewold12. Engineering of Tomato Glandular Trichomes for the Production of Specialized MetabolitesRuy W.J. Kortbeek, Jiesen Xu, Aldana Ramirez, Eleni Spyropoulou, Paul Diergaarden, Ilona Otten-Bruggeman, Michiel de Both, Raimund Nagel, Axel Schmidt, Robert C. Schuurink and Petra M. Bleeker13. Tomato Fruits – a Platform for Metabolic Engineering of TerpenesMichael Gutensohn, and Natalia Dudareva14. Libraries of Synthetic Tale-Activated Promoters: Methods and ApplicationTom Schreiber and Alain Tissier

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience