Structural Genomics

General Applications

Häftad, Engelska, 2021

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This updated edition emphasizes the benefits of structural genomics to the wider structural research community with a diverse range of applicable methods.

Produktinformation

Utgivningsdatum2021-11-01
Mått178 x 254 x 21 mm
Vikt713 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor364
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071608944

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Kemi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

High-Throughput Protein Engineering by Massively Parallel Combinatorial Mutagenesis.- Rational Design and Construction of Active-Site Labeled Enzymes.- Screening and Production of Recombinant Human Proteins: Ligation Independent Cloning.- Screening and Production of Recombinant Human Proteins: Protein Production in E. coli.- Screening and Production of Recombinant Human Proteins: Protein Production in Insect Cells.- Expression Screening of Human Integral Membrane Proteins Using BacMam.- High-Throughput Expression Screening in Mammalian Suspension Cells.- In Vitro Production of Perdeuterated Proteins in H2O for Biomolecular NMR Studies.- Minimizing Heterogeneity of Protein Samples for Metal Transporter Proteins Using SAXS and Metal Radioisotopes.- Hydrogen/Deuterium Exchange-Mass Spectrometry for Probing Changes in Conformation and Dynamics of Proteins.- BeStSel: From Secondary Structure Analysis to Protein Fold Prediction by Circular Dichroism Spectroscopy.- Navigating the Global Protein-Protein Interaction Landscape Using iRefWeb.- State-of-the-Art Data Management: Improving the Reproducibility, Consistency, and Traceability of Structural Biology and In Vitro Biochemical Experiments.- Protein Structure Modeling with MODELLER.- Parameterization of a Dioxygen Binding Metal Site Using the MCPB.py Program.- Parameterization of Large Ligands for Gromacs Molecular Dynamics Simulation with LigParGen.- Simulation of Proteins Modified with a Fluorescent Label.- Protocol for Simulations of PEGylated Proteins with Martini 3.- Molecular Data Visualization on Mobile Devices: A Quick Starter’s Guide.- Molecular Data Visualization with Augmented Reality (AR) on Mobile Devices.