Separation of Enantiomers by Capillary Electrophoresis

The author is a development scientist in pharmaceutical and analytical research and development at AstraZeneca in Macclesfield. He has a general interest in separation science and has been active for a number of years in the field of research into the principles and application capillary electrophoresis in pharmaceutical analysis.

• Supplement Vol. 54, 2001.- 1 Enantiomers and Separation.- 1.1 Introduction.- 1.2 Enantiomers and Living Organisms.- 1.3 The Separation of Enantiomers.- 1.4 Electrophoretic Separation of Enantiomers.- 1.5 Enantiomer Separations by Capillary Electrophoresis.- 1.6 Conclusion.- 2 The Principles of Separation in CE.- 2.1 Introduction: Movement and Separation.- 2.2 Separative and Non-Separative Transport in CE.- 2.3 Electrophoretic Mobility.- 2.4 Electroosmotic Mobility.- 2.5 CE Instrumentation.- 2.6 Conclusion.- 3 Modelling Enantiomer Separation by CE.- 3.1 The Use of Models.- 3.2 Background.- 3.3 The Physical Model.- 3.4 A Basic Mathematical Model.- 3.5 Extended Theoretical Models.- 3.6 Conclusion.- 4 Method Development Strategies.- 4.1 The Purpose of the Method.- 4.2 Factors Controlling Resolution.- 4.3 The Structure of the Analyte.- 4.4 The Choice of Chiral Selector.- 4.5 Other Separation Conditions.- 4.6 Optimisation Approaches.- 4.7 Validation.- 4.8 Computer Simulations.- 4.9 Conclusion.- 5 The Use of Cyclodextrins as Chiral Selectors.- 5.1 Introduction.- 5.2 Structure and Properties.- 5.3 Cyclodextrin Production.- 5.4 Complexation Mechanisms.- 5.5 Cyclodextrin Classes.- 5.6 Analyte Classes.- 5.7 Analyte Structure.- 5.8 Batch and Source Variation.- 5.9 Conclusion.- 6 Other Chiral Selectors.- 6.1 Introduction.- 6.2 Ligand Exchange Selectors.- 6.3 Chiral Surfactants.- 6.4 Alkaloids.- 6.5 Crown Ethers.- 6.6 Natural Macromolecules.- 6.7 Non-Aqueous CE.- 6.8 Derivatisation.- 6.9 Capillary Electrochromatography.- 6.10 Conclusion.