Schmidtea Mediterranea

Methods and Protocols

Häftad, Engelska, 2024

2 599 kr

Beställningsvara. Skickas inom 10-15 vardagar

Fri frakt för medlemmar vid köp för minst 249 kr.

Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Produktinformation

Utgivningsdatum2024-07-12
Mått178 x 254 x 16 mm
Vikt554 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor280
FörlagSpringer-Verlag New York Inc.
ISBN9781071632772

Tillhör följande kategorier

Building Phylogenies from Transcriptomic Data.- Isolation and Library Preparation of Planarian piRNAs.- Genome-Wide Analysis of Planarian piRNAs.- Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians.- Colorimetric Whole-Mount In Situ Hybridization in Planarians.- Single Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians.- Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.- Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry.- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells.- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells.- Papain-Based Dissociation of Schmidtea mediterranea Cells.- Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells.- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species.- A Planarian Model System to Study Host-Pathogen Interactions.- TUNEL Staining in Sections of Paraffin-Enabled Planarians.- Quantitative Analysis of Planarian Pigmentation.- mRNA Transfection of S. mediterranea Cells for Luminescence Analysis.