SARS-CoV-2

Methods and Protocols

Inbunden, Engelska, 2022

Av Justin Jang Hann Chu, Bintou Ahmadou Ahidjo, Chee Keng Mok

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This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe.

Produktinformation

Utgivningsdatum2022-05-14
Mått178 x 254 x 37 mm
Vikt1 110 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor478
FörlagSpringer-Verlag New York Inc.
ISBN9781071621103

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik

Evolution and Epidemiology of SARS-CoV-2 Virus.- Molecular Epidemiology of SARS-CoV-2 by Sequencing.- Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2.- Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance.- Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens.- Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay.- Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans.- Propagation and Quantification of SARS-CoV-2.- Quantification of Infectious SARS‐CoV‐2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay.- One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification.- Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics.- Detection of SARS-CoV-2 by Mass Spectrometry.- Bioinformatics-Based Approaches to Study Virus–Host InteractionsDuring SARS-CoV-2 Infection.- Human Nasal Epithelial Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2.- Animal Models of COVID-19: Nonhuman Primates.- Animal Models of COVID-19: Transgenic Mouse Model.- Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and Multiple Immunohistochemistry.- Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses.- Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach.- Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test.- Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2.- Cytopathic Effect (CPE)-Based Drug Screening Assay for SARS-CoV-2.- Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2.- Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility.- Methods of SARS-CoV-2 Inactivation.