Salamanders

Methods and Protocols

Inbunden, Engelska, 2022

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This volume provides a comprehensive reference for researchers aiming to bring new techniques and approaches to their scientific research using urodeles.

Produktinformation

Utgivningsdatum2022-10-23
Mått178 x 254 x 33 mm
Vikt1 128 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor483
FörlagSpringer-Verlag New York Inc.
ISBN9781071626580

Tillhör följande kategorier

Salamanders as key models for development and regeneration research.- Establishing a new research axolotl colony.- Health monitoring for laboratory salamanders.- Husbandry, captive breeding and field survey of Chinese giant salamander (Andrias davidianus).- Whole mount in situ hybridization (WISH) for salamander embryos and larvae.- Hybridization chain reaction fluorescence in situ hybridization (HCR-FISH) in Ambystoma mexicanum tissue.- Ethyl cinnamate-based tissue clearing strategies.- Induction and characterization of cellular senescence in salamanders.- Methods for studying appendicular skeletal biology in axolotls.- Fluorescence in situ hybridization of DNA-probes on mitotic chromosomes of the Mexican axolotl.- The use of small molecules to dissect developmental and regenerative processes in axolotls.- COMET Assay for detection of DNA damage during axolotl tail regeneration.- In vivo and Ex vivo View of Newt Lens Regeneration.- Bead implantation and delivery of exogenous growth factors.- The Accessory Limb Model regenerative assay and its derivatives.- Embryonic Tissue and Blastema Transplantations.- Retinoic acid-induced limb duplications.- Isolation and characterization of peritoneal macrophages from salamanders.- Navigation and use of custom tracks within the axolotl genome browser.- Chromosome Conformation Capture for large genomes.- Axolotl transgenesis via injection of I-SceI meganuclease or Tol2 transposon system.- A practical guide for CRISPR-Cas9-induced mutations in axolotls.- Applying a knock-in strategy to create reporter tagged knockout alleles in axolotls.- Baculovirus production and infection in axolotls.- Cell dissociation techniques in salamanders.- Axolotl metabolism: measuring metabolic energy.- Artificial insemination in axolotls.- Screening salamanders for symbionts.- Assessing leukocyte profiles of salamanders and other amphibians: a herpetologists’ guide.- Best practices to promote data utility and recycling by the salamander community.- Now that we got there, what's next?.