RT-PCR Protocols

• The RT-PCR Detective: Hunting Down the Best Method.- Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology.- Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer.- Transcriptome Profiling of Host–Microbe Interactions by Differential Display RT-PCR.- Quantitative RT-PCR Methods for Mature microRNA Expression Analysis.- Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction.- Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR.- Simultaneous Detection of Bluetongue Virus RNA, Internal Control GAPDH mRNA, and External Control Synthetic RNA by Multiplex Real-Time PCR.- Detection of West Nile Viral RNA from Field-Collected Mosquitoes in Tropical Regions by Conventional and Real-Time RT-PCR.- Detection of Antisense RNA Transcripts by Strand-Specific RT-PCR.- RT-PCR Amplification and Cloning of Large Viral Sequences.- The RT-PCR Mathematician: Assessing Gene and RNA Expression.- One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification.- Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR.- Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction.- The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes.- The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification.- The RT-PCR Master Chef: Finding the Right Ingredients.- Skeletal Muscle RNA Extraction in Preparation for RT-PCR.- Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay.- Primer Design for RT-PCR.- Hot Start PCR.- Real-time RT-PCR for Automated Detection ofHIV-1 RNA During Blood Donor Screening.

From the reviews of the second edition:“The second edition of RT-PCR protocols and attempts to cover the most commonly used RT-PCR protocols, giving both beginners and experts a deeper understanding of the experimental set-up. … There are often flow diagrams or pictures to aid understanding and the references are helpful, if you require additional information. … a good investment for any lab interested in ensuring that their RT-PCR reactions are as productive and well-controlled as possible. … Overall a useful and detailed text written in an informative and helpful style.” (Hayley Evans, Immunology News, May, 2011)