RNA Therapeutics

This book provides an overview of RNA-based technologies, covering their mechanisms, therapeutic applications, and challenges. Chapters explore therapeutic RNAs, RNA stability, mRNA vaccine formulation, strategies to overcome immunogenicity, delivery hurdles, mRNA-based cancer immunotherapy, CAR and TCR T cell therapies, and RNA interference. Additional chapters examine RNA delivery systems such as lipid nanoparticles, nanotubular structures and extracellular vesicles. Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions tothe material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlightstips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Therapeutics: From siRNA to mRNA Innovations aims to be comprehensive guide forresearchers in the field.