RNA Spectroscopy

Methods and Protocols

Häftad, Engelska, 2021

Av Véronique Arluison, Frank Wien, Veronique Arluison

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This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins.

Produktinformation

Utgivningsdatum2021-02-01
Mått178 x 254 x 20 mm
Vikt692 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor356
FörlagSpringer-Verlag New York Inc.
ISBN9781071602805

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Encapsulation of Fluorescently Labelled RNAs into Surface-Tethered Vesicles for Single-Molecule FRET Studies in TIRF Microscopy.- Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein Complexes.- Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species.- Dynamic Light Scattering Analysis on RNA Associated to Proteins.- Bioinformatic Application of Fluorescence-Based In Vivo RNA Regional Accessibility Data to Identify Novel sRNA Targets.- Surface Plasmon Resonance for Investigating Molecular Interactions with RNA.- RNA folding and Unfolding Under Force: Single-Molecule Experiments and their Analysis.- Mapping of Post-Transcriptional tRNA Modifications by Two-Dimensional Gel Electrophoresis Mass Spectrometry.- Native Electrospray Ionization Mass Spectrometry of RNA-Ligand Complexes.- Application of FTIR Spectroscopy to Analyse RNA Structure.- Application of Synchrotron Radiation Circular Dichroism for RNA Structural Analysis.- Application of NIR Raman Spectroscopy to Probe the Flexibility of RNA Structure.- Small Angle Neutron Scattering of RNA-Protein Complexes.- Structural Analysis of RNA by Small-Angle X-Ray Scattering.- Site-Specific Spin-Labeling of RNA for NMR and EPR Structural Studies.- Analysis of the HIV-1 Genomic RNA Dimerization Initiation Site Binding to Aminoglycoside Antibiotics using Isothermal Titration.- Use of Fluorescent Nucleotides to Map RNA-Binding Sites on Protein Surface.- Fluorescent Oligonucleotide Probes for the Quantification of RNA by Real-Time qPCR.- Probing RNA-Protein Interactions and RNA Compaction by Sedimentation Velocity Analytical Ultracentrifugation.- RNA Nanostructures Molecular Imaging.- Grad-CryoEM: Tool to Isolate Translation Initiation Complexes from Rabbit Reticulocyte Lysate Suitable for Structural Studies.- Application of NMR Spectroscopy to Determine Small RNA Structure.