RNA Scaffolds

Methods and Protocols

Häftad, Engelska, 2022

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This second edition volume expands on the previous edition with discussions of recently developed techniques that use RNA scaffolds as molecular tools.

Produktinformation

Utgivningsdatum2022-06-06
Mått178 x 254 x 17 mm
Vikt576 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor288
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071615010

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Kemi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Predicting RNA Scaffolds with a Hybrid Method of Vfold3D and VfoldLA.- RNA Footprinting Using Small Chemical Reagents.- Improving RNA Crystal Diffraction Quality by Post-Crystallization Treatment.- Using tRNA Scaffold to Assist RNA Crystallization.- RNA Modeling with the Computational Energy Landscape Framework.- Co-Expression and Co-Purification of RNA-Protein Complexes in Escherichia coli.- In Vivo Production of Small Recombinant RNAs Embedded in 5S rRNA-Derived Protective Scaffold.- Production of Circular Recombinant RNA in Escherichia coli Using Viroid Scaffolds.- Identification of RNA-Binding Proteins Associated to RNA Structural Elements.- Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.- Rational Design of Allosteric Fluorogenic RNA Sensors for Cellular Imaging.- Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria.- FRET-Analysis of RNA-Protein-Interactions using Spinach-Aptamers.- Engineering Aptazyme Switches for Conditional Gene Expression in Mammalian Cells Utilizing an In Vivo Screening Approach.- Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells.- Folding RNA–Protein Complex into Designed Nanostructures.- An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.- Expression and Purification of tRNA/pre-miRNA-Based Recombinant Noncoding RNAs.- Synthetic Biology Medicine and Bacteria-Based Cancer Therapeutics.