Recombinant Glycoproteins

Methods and Protocols

Häftad, Engelska, 2025

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Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins.

Produktinformation

Utgivningsdatum2025-03-31
Mått178 x 254 x 21 mm
Vikt706 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor355
FörlagSpringer-Verlag New York Inc.
ISBN9781071636688

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Biokemi inom Naturvetenskap och teknik

Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells.- Production and Purification of Filovirus Glycoproteins.- Modification of N-Linked Glycan Sites in Viral Glycoproteins.- Production of Influenza Virus Glycoproteins Using Insect Cells.- SARS-CoV-2 S-Protein – Ace2 Binding Analysis Using Surface Plasmon Resonance.- A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain.- Production and Purification of Plasmodium Circumsporozoite Protein in Lactococcus lactis.- Analysis of Caenorhabditis Protein Glycosylation.- Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates.- Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors.- Production and Purification of Antibodies in Chinese Hamster Ovary Cells.- Mammalian Antigen Display for Pandemic Countermeasures.- Analysisof Native and Permethylated N-Glycan Isomers Using MGC-LC-MS Techniques.- Targeted Glycoproteomics Analysis Using MRM/PRM Approaches.- Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches.- Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A.- O-Glycoproteomics Sample Preparation and Analysis Using NanoHPLC and Tandem MS.- Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers.- Cell-Free Systems for the Production of Glycoproteins.- Considerations for Glycoprotein Production.