Protocols for Macroalgae Research
Inbunden, Engelska, 2018
Av Bénédicte Charrier, Thomas Wichard, C R K Reddy, France) Charrier, Benedicte (CNRS Station Biologique
3 409 kr
Produktinformation
- Utgivningsdatum2018-04-26
- Mått156 x 234 x 38 mm
- Vikt1 238 g
- SpråkEngelska
- Antal sidor496
- FörlagTaylor & Francis Inc
- EAN9781498796422
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Dr. Bénédicte Charrier is a senior scientist from CNRS working at Station Biologique Roscoff, France. She is a specialist of macroalgae morphogenesis and development, with an expertise on the filmentous alga Ectocarpus siliculosus. She is currently the Chair of the European network "Phycomorph", supported by the Cooperation in Science & Technology (COST) Association (2015-2019), which aims to coordinate research in growth, reproduction, and morphogenesis of macroalgae in Europe and in association with Asian and North American laboratories.Dr. Thomas Wichard is a research group leader at the Institute for Inorganic and Analytical Chemistry of the Friedrich Schiller University Jena. The main focus of his research group is to elucidate the mutualistic interactions between bacteria and the marine macroalga Ulva ("cross-kingdom-cross-talk"). The group applies various methodologies in analytical chemistry, chemical ecology and molecular biology to understand the basis of eco-physiological processes.Dr. CRK Reddy is a Chief Scientist in Seaweed Biology and Biotechnology Cultivation at CSIR-Central Salt and Marine Chemicals Research Institute in Bhavnagar, India. He is an expert in marine macroalgae, making significant contributions to macroalgae tissue culture and protoplast techniques including genetic improvement of seaweeds, seaweed biomass for chemicals and biofuels, seaweed biodiversity, and seaweed biology and cultivation.
- CONTENTSSection I: Cultivating and preserving seaweedsChapter 1 Seaweed in high-energy environments: Protocolto move Saccharina cultivation offshoreChapter 2 Cultivation protocol for Saccharina latissimaChapter 3 Derivation of clonal stock cultures and hybridizationof kelps: A tool for strain preservation and breedingprogramsChapter 4 Cryopreservation of macroalgaeChapter 5 Unraveling seaweeds bacteriomes: From field siteto computer screenChapter 6 Heavy metal ecotoxicity on the early life historystages of macroalgaeChapter 7 A simple protocol for a rapid and consistentproduction of a large number of viable protoplastsfrom the Ulvophycean speciesChapter 8 Purification of sporulation and swarming inhibitorsfrom Ulva: Application in algal life-cycle controllingChapter 9 Preparation of axenic cultures in Ulva (Chlorophyta)Section II: Chemical compositionChapter 10 Biochar production from seaweedsChapter 11 Identification and quantification of laminarinsin brown algaeof macroalgaeChapter 12 Determination of carbohydrate compositionChapter 13 Quantification of proteins in seaweedsChapter 14 Comprehensive phytohormone quantificationin the red alga Pyropia yezoensis by liquidchromatography–mass spectrometryChapter 15 Total phenolic content and antioxidant capacityanalysis of seaweed biomassChapter 16 Extraction of phycocyanin and phycoerythrin pigmentsChapter 17 Quantification and localization of reactive oxygenspecies in marine macrophytesChapter 18 Metabolomics of intra- and extracellular metabolitesfrom micro- and macroalgae using GC–MSand LC–MSChapter 19 Preparative extraction of exometabolitesfrom seaweed surfacesFlorian WeinbergerChapter 20 Disruption-free solid-phase extraction of surfacemetabolites from macroalgaeEmilio Cirri and Georg PohnertSection III: Cellular and molecular characterizationChapter 21 The immunodetection and in situ imaging ofcell-wall polysaccharides in brown algaeChapter 22 Atomic force microscopy based analysis of cell-wallelasticity in macroalgaeChapter 23 Dynamic and microscale mapping of cell growth:Case of Ectocarpus filament cellsChapter 24 Actin fluorescent staining in the filamentous brownalga Ectocarpus siliculosusChapter 25 Cryofixation of brown algae for transmissionelectron microscopyChapter 26 Probing the subcellular topography ofseaweeds: Transmission electron microscopy,immunocytochemistry, and correlative lightmicroscopyChapter 27 Coralline algae preparation for scanning electronmicroscopy and optical microscopyChapter 28 Extraction of high quality RNA from brown algaefor transcriptomic analysisChapter 29 Induction of sexual reproduction in Spirogyracultures for laser capture microdissection of gametesand zygotesChapter 30 Cloning and expression strategies for thepostgenomic analysis of brown algaeChapter 31 Polyethylene glycol-mediated transformation inthe green macroalga Ulva mutabilis (Chlorophyta):A forward genetics approachIndex
"This book is an impressive picture of the tremendous evolution of phycology during the ten past years. Each PhD student and postdoctoral researcher should have it in its laboratory; he will find in it the basic most recent protocols ready to use for his study, and the matter for imaginative, limitless experiments."—Bruno de Reviers, Muséum National d’histoire Naturelle, Paris, France""This book is a well written and informative guide to methods for macroalgal (seaweed) culture and analysis. It is notable for the broad coverage of macroalgal biology, from culture approaches, through methods for compositional analysis to the newer molecular approaches of metabolomics and transcriptomics. It will certainly prove to be a useful resource for those involved in macroalgal culture and biotechnology."—John Beardall, Monash University, Melbourne, Victoria, AustraliaProtocols for Macroalgae Research describes state-of-the-art approaches and applications in seaweed research. The book is comprised of 31 protocols in all and provides critical information regarding the cultivation and preservation of seaweeds, their chemical composition, as well as their cellular and molecular characterization. This type of research is critical and timely, as the last few decades have witnessed remarkable advancements in phycological research worldwide. Consideration of algal resources for food, fuel, chemicals, feed, and fertilizer has furthered the development of diverse laboratory techniques that not only advance their utilization substantially but also provided new insights in understanding the molecular basis of their form and function. This recent progress has enabled researchers to determine and quantify various metabolites and organic macromolecules such as carbohydrates, proteins, and lipids. Protocols for Macroalgae Research thus uses macroalgae as a model organism for addressing fundamental research problems. No recent comprehensive literature of this format exists on this topic. As such, this state-of-the-art protocol text both develops and standardizes the various macroalgae approaches located within the field. It does so by featuring a wide-range of protocols from invited experts; each protocol features a brief introductory overview, an inventory of materials and reagents, and step-by-step experimental procedures which include helpful notes offering care and cautions. This protocol book is an invaluable reference for beginning and preexisting researchers within the field of macroalgae.