Proteomic Profiling

Bio-Rad Laboratories GmbH, 80939 München, Heidemannstr. 164 Sitz der Gesellschaft: München - Amtsgericht München HRB 7491

• Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization.- Sample Preservation Through Heat Stabilization of Proteins: Principles and Examples.- Isolating Peripheral Lymphocytes by Density Gradient Centrifugation and Magnetic Cell Sorting.- Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling.- Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an in vitro Exercise Model.- Urinary Pellet Sample Preparation for Shotgun Proteomic Analysis of Microbial Infection and Host Pathogen Interactions.- A Protocol for the Parallel Isolation of Intact Mitochondria from Rat Liver, Kidney, Heart and Brain.- Isolation of Mitochondria from Cultured Cells and Liver Tissue Biopsies for Molecular and Biochemical Analyses.- Dynamic Range Compression with ProteoMiner: Principles and Examples.- Qualitative and Quantitative Proteomic Analysis of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue.- Full-Length Protein Extraction Protocols and Gel-Based Downstream Applications in Formalin-Fixed Tissue Proteomics.- Enrichment of Low-Abundant Protein Targets by Immunoprecipitation Upstream of Mass Spectrometry.- P Isolation of Extracellular Vesicles for Proteomic Profiling.- A Protocol for Exosome Isolation and Characterization: Evaluation of Ultracentrifugation, Density-Gradient Separation, and Immunoaffinity Capture Methods.- Chloroplast Isolation and Affinity Chromatography for Enrichment of Low Abundant Proteins in Complex Proteomes.- Depletion of RuBisCO Protein Using the Protamine Sulfate Precipitation Method.- Step by Step Preparation of Proteins for Mass Spectrometric Analysis.- Identification of Protein N-Termini Using TMPP or Dimethyl Labeling and Mass Spectrometry.- Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS.- Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation and N-Linked Sialylated Glycosylation.- Protein Profiling and Phosphoprotein Analysis by Isoelectric Focusing.- Principles and Examples of Gel-Based Approaches for Phosphoprotein Analysis.- Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation.- Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry.- In-Gel Peptide IEF Sample Preparation for LC/MS Analysis.- Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technology.- 2-D Western Blotting for Evaluation of Antibodies Developed for Detection of Host Cell Protein.- Free Flow Electrophoresis for Separation of Native Membrane Protein Complexes.- Three-Dimensional Electrophoresis for Quantitative Profiling of Complex Proteomes.- A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of β-Catenin.- Identification of SUMO E3 Ligase Specific Substrates Using the HuProt Human Proteome Microarray.- Amyloid-Binding Proteins: Affinity Based Separation, Proteomic Identification and Optical Biosensor Validation.- Proteomic Profiling by Nanomaterials-Based Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for High-Resolution Data and Novel Protein Information Directly from Biological Samples.