Proteoglycans

Methods and Protocols

Inbunden, Engelska, 2023

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This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs.

Produktinformation

Utgivningsdatum2023-01-21
Mått178 x 254 x 24 mm
Vikt823 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor317
FörlagSpringer-Verlag New York Inc.
ISBN9781071629451

Tillhör följande kategorier

Biokemi inom Naturvetenskap och teknik

Detection of Glycosaminoglycans in Biological Specimens.- Immunochemical Detection and Glycosaminoglycan Disaccharide-Based Characterization Of Chondroitin Sulfate Proteoglycans.- Proteoglycans: A Tool for detecting hyaluronan by ELISA-like Methods.- Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix.- Urinary Glycosaminoglycans: Characterization and Quantification.- HPLC-MS characterization of tissue-derived heparan sulfate and chondroitin sulfate.- Evaluation of Glycosaminoglycans Biological Age in Cells and Tissues.- Studying the effects of Glycosaminoglycans in Cell Morphological Aspect With Scanning Electron Microscopy.- Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity.- Analysis of Extracellular Vesicle-associated Proteoglycans.- Analysis of Aggrecan Glycanation by Western Blot in Cell Culture.- Repulsive Scaling Replica Exchange Molecular Dynamics in ModelingProtein-Glycosaminoglycan Complexes.- In vitro investigation of hyaluronan/CD44 network.- Separation and Identification of Native Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and Immunoblotting.- Building, Visualizing, and Analyzing Glycosaminoglycan-Protein Interaction Networks.- An Enzymatic Activity Assay for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors and Studying Kinetics.- A method for Urinary Trypsin Inhibitor (UTI) purification combining anion-exchange chromatography enrichment and preparative SDS-PAGE.- Enzymatic Sequencing of Heparin Oligosaccharides Using Exolyase.- Evaluating the Effects of microRNAs on Proteoglycans and Matrix Constituents’ Expression and Functional Properties.- Proteoglycan expression studied by microRNAs.- Modeling and simulations of Extra Cellular Glycoproteins.