Protein Design

Protein Design: Method and Applications, Second Editionexpands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters onmetals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive,Protein Design: Method and Applications, Second Editionprovides a number of practical protocols and instructive reviews to aid in the creation of new experiments.