Protein Arginylation

Methods and Protocols

Häftad, Engelska, 2016

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This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes.

Produktinformation

Utgivningsdatum2016-10-22
Mått178 x 254 x 9 mm
Vikt351 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor149
FörlagHumana Press Inc.
ISBN9781493945689

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Biokemi inom Naturvetenskap och teknik

Protein Arginylation: Over 50 Years of Discovery.- Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase.- Arginyltransferase: A Personal and Historical Perspective.- Arginylation in a Partially Purified Fraction of 150k xg Supernatants of Axoplasm and Injured Vertebrate Nerves.- Preparation of ATE1 Enzyme from Native Mammalian Tissues.- Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging.- Assaying the Post-Translational Arginylation of Proteins in Cultured Cells.- Assaying ATE1 Activity in Yeast by β-gal Degradation.- Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays.- Assaying ATE1 Activity In Vitro.- High Throughput Arginylation Assay in Micro plate Format.- Assay of Arginyltransferase Activity by a Fluorescent HPLC Method.- Identification of Arginylated Proteins by Mass Spectrometry.- Analysis of Arginylated Peptides by Subtractive Edman Degradation.- Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles.- Applying Arginylation for Bottom-Up Proteomics.- Development of New Tools for the Studies of Protein Arginylation.