Prokaryotic Gene Regulation

Methods and Protocols

Inbunden, Engelska, 2022

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This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both.

Produktinformation

Utgivningsdatum2022-08-04
Mått178 x 254 x 24 mm
Vikt816 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor318
FörlagSpringer-Verlag New York Inc.
ISBN9781071624128

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

Chromosome Conformation Capture in Bacteria and Archaea.- Micrococcal Nuclease Digestion Assays for the Analysis of Chromosome Structure in Archaea.- Detecting DNA Methylations in the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius using SMRT-Sequencing.- ProD: A Tool for Predictive Design of Tailored Promoters in Escherichia coli.- Computational Study on the Dynamics of Mycobacterium Tuberculosis RNA Polymerase Assembly.- In Vitro Transcription Assay for Archaea Belonging to Sulfolobales.- Prediction of DNA-Binding Transcription Factors in Bacteria and Archaea Genomes.- In Vivo Screening Method for the Identification and Characterization of Prokaryotic, Metabolite-Responsive Transcription Factors.- Application of Special Electron Microscopy Techniques to the Study of DNA-Protein Complexes in E. coli Cells.- High-Speed Atomic Force Microscopy Visualization of Protein-DNA Interactions using DNA Origami Frames.- Separation and Characterization of Protein-DNA Complexes by EMSA and In-Gel Footprinting.- Chemical Protection and Premodification Binding Interference for the Identification of Phosphate and Base-Specific Contacts in Protein-DNA Complexes.- Analysis of Protein-DNA Interactions using Isothermal Titration Calorimetry: Successes and Failures.- Determination of RNA Structure with In Vitro SHAPE-Experiments.- Microscale Thermophoresis to Study RNA-RNA Binding Affinity.- Toeprint Assays for Detecting RNA Structure and Protein-RNA Interactions.