Practical Guide to Sperm Analysis

Nicolas Garrido, PhD, MSc is the Director of the Andrology Laboratory and Sperm Bank at Instituto Valenciano de Infertilidad (IVI), Spain, and coordinates the fellowship courses for the Teaching Area in the IVI group. Rocio Rivera works with him as Supervisor of the Andrology Laboratory and Sperm Bank at IVI.

• The usefulness of sperm viability testing in reproductive technology: The hypo-osmotic swelling test, laser and motility stimulants. History and physical examination: Male infertility. The role of hormonal profiles to forecast male fertility chances. The application of genetic tests in an Assisted Reproduction Unit: Karyotype. Genetic testing of Y chromosome microdeletion. The application of genetic tests in an assisted reproduction unit: Sperm FISH. The application of genetic tests in an assisted reproduction unit: cystic fibrosis carrier screening. The application of genetic tests in an assisted reproduction unit: MRNA microarrays. The application of genetic tests in an Assisted Reproduction Unit: DNA fragmentation. The application of genetic tests in an Assisted Reproduction Unit: DNA methylation defects. The use of sperm ultramorphology assessment in assisted reproduction. The usefulness of antisperm antibodies testing. The measurement of oxidative stress in semen and use in Assisted Reproduction. The importance of sperm surface markers in reproductive success: Sperm hyaluronan binding. The importance of sperm surface markers in reproductive success: Surface change. The relevance in reproductive success of sperm head polarization (birefringence). The applicability of Raman spectroscopy in sperm diagnosis and selection for assisted reproduction. The use of sperm proteomics in the Assisted Reproduction laboratory. The evaluation of surgically retrieved spermatozoa and its usefulness in Assisted Reproduction.