Practical Fluorescence Spectroscopy

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applicationsPresents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretationsCovers steady-state phenomena, time-resolved phenomena, and advanced methodsSpans biophysical, biochemical, and biomedical applicationsDescribes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements