Plastids

Methods and Protocols

Inbunden, Engelska, 2024

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This second edition volume expands on the previous edition with new and updated chapters that explore our current view on plastid evolution, structure, and function.

Produktinformation

Utgivningsdatum2024-03-20
Mått178 x 254 x 24 mm
Vikt831 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor322
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071637258

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How Did Thylakoids Emerge in Cyanobacteria and How Were the Primary Chloroplast and Chromatophore Acquired?.- Complex Endosymbioses I: From Primary to Complex Plastids, Serial Endosymbiotic Events.- Complex Endosymbiosis II: The Non-Photosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism.- Diversification of Plastid Structure and Function in Land Plants.- The Main Functions of Plastids.- Plastid Transient and Stable Interactions with Other Cell Compartments.- Purification of Chloroplast Envelope, Thylakoids, and Stroma from Angiosperm Leaves.- Isolation of the Inner and Outer Membranes of the Chloroplast Envelope from Angiosperms.- Analysis of a Super-Complex at Contact Sites between Mitochondria and Plastids.- Isolation of High-Quality Plastids from the Diatom Phaeodactylum tricornutum.- Determining the Subcellular Localization of Proteins in the Different Membranes of Diatom Secondary Plastid.- Monitoring of Lipid Fluxes between Host and Plastid-Bearing Apicomplexan Parasites.- Extraction and Quantification of Lipids from Plant or Algae.- Quantitative Assessment of the Chloroplast Lipidome.- The Use of Nanopore Sequencing to Analyze the Chloroplast Transcriptome Part I: Library Preparation.- The Use of Nanopore Sequencing to Analyze the Chloroplast Transcriptome Part II: Bioinformatic Analyzes and Virtual RNA Blots.- Targeted Gene Editing of Nuclear-Encoded Plastid Proteins in Phaeodactylum tricornutum via CRISPR/Cas9.- Isolation of Cytosolic Ribosomes Associated with Plant Mitochondria and Chloroplasts.- A Practical Guide to the Representation of Protein Regulation in the Web Application ChloroKB.