Plant Genome Engineering

Methods and Protocols

Häftad, Engelska, 2023

Av Bing Yang, Wendy Harwood, Qiudeng Que

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The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering.

Produktinformation

Utgivningsdatum2023-03-31
Mått178 x 254 x 22 mm
Vikt757 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor388
FörlagSpringer-Verlag New York Inc.
ISBN9781071631331

Tillhör följande kategorier

Botanik inom Naturvetenskap och teknik
Växtböcker inom Djur och natur
Biovetenskap inom Naturvetenskap och teknik

PAM-Less CRISPR-SpRY Genome Editing in Plants.- Type I-D CRISPR System-Mediated Genome Editing in Plants.- CRISPR/LbCas12a-Mediated Genome Editing in Soybean.- Base Editing in Poplar through an Agrobacterium-Mediated Transformation Method.- Genetic Engineering of Potato (Solanum tuberosum) Chloroplasts using the Small Synthetic Plastome “Mini-Synplastome”.- Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout using CRISPR-BETS.- Construction of CRISPR/Cas9 Multiplex Genome Editing System in Rice.- Use of Fluorescent Protein Reporters for Assessing and Detecting Genome Editing Reagents and Transgene Expression in Plants.- Automated, High-Throughput Protoplast Transfection for Gene Editing and Transgene Expression Studies.- Delivery of CRISPR-Cas12a Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell Line.- Transgene-Free Genome Editing in Nicotiana benthamiana with CRISPR/Cas9 Delivered by a Rhabdovirus Vector .- Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.).- Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves.- Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.- Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method.- Efficient Targeted Mutagenesis in Brassica Crops using CRISPR/Cas Systems.- Introduction of Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean (Glycine max (L.) Merr.).- A CRISPR/Cas9 Protocol for Target Gene Editing in Barley.- Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration.- Stepwise Optimization of Real-Time RT-PCR Analysis.- CRISPR/Cas9 Technology for Potato Functional Genomics and Breeding.- Recent Advances in Engineering of In Vivo Haploid Induction Systems.