Organelle Proteomics

Proteomic analyses have proven to be an invaluable tool inobtaining high-throughput protein identification from low-abundance, complex biological samples. In Organelle Proteomics, detailed protocols providestep-by-step instructions tosuccessfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well asby preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomicsallowsscientists to gain a vital and importantunderstanding of organelle study.