Del 438 - Methods in Molecular Biology

Neural Stem Cells

Methods and Protocols

Inbunden, Engelska, 2008

Av Leslie P. Weiner

1 459 kr

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Although there has been an explosion of interest and technology in the study of neural stem cells, many questions related to stem cell properties and neural stem cell lineage and differentiation still linger. Neural Stem Cells, 2nd Edition revises and expands upon the successful first edition in order to provide the most current, cutting-edge methods of today for the scientists working to answer these questions. The use of these step-by-step, readily reproducible laboratory protocols will allow investigators to produce pure populations that can serve as a means of understanding the biology of neural stem cells and adapting them for transplantation into disease models. In addition to the topics covered in the first edition, this new volume provides recently developed alternative methods for both human and rodent NSCs, along with chapters on karyotyping, migration, apoptosis, and NSC markers. Following the Methods in Molecular Biology™ series format, each chapter has an extended introduction for background, a list of necessary materials, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and current, Neural Stem Cells, 2nd Edition is an excellent source of information and inspiration for scientists longing to use these powerful cells against neurogenetic and neurodegenerative diseases.

Produktinformation

Utgivningsdatum2008-02-01
Mått155 x 235 x 31 mm
Vikt883 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor406
Upplaga2
FörlagHumana Press Inc.
ISBN9781588298461

Tillhör följande kategorier

Anatomi inom Medicin
Antropologi inom Samhalle och politik
Biovetenskap inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
Neurokirurgi inom Medicin

Definitions and Criteria for Stem Cells.- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard.- Isolation and Culture of NSCs.- Neural Differentiation of Human Embryonic Stem Cells.- Generating Neurons from Stem Cells.- Isolation of Neural Stem and Precursor Cells from Rodent Tissue.- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization.- Adult Neural Stem Cells.- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb.- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium.- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property.- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood.- Production of Neurospheres from CNS Tissue.- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells.- In Vitro Studies: A. Cellular Techniques.- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures.- Telomere Neurobiology.- Chromosomal Mosaicism in Neural Stem Cells.- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype.- In Vitro Migration Assays of Neural Stem Cells.- Measuring Apoptosis in Neural Stem Cells.- Markers of Adult Neural Stem Cells.- In Vitro Studies: B. Molecular Techniques.- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR.- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells.- Electroporation Strategies for Genetic Manipulation and Cell Labeling.- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches.- Studies for Transplantation.- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo.- Immunocytochemical Analysis of Neuronal Differentiation.- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold.- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS.- Optimizing Stem Cell Grafting into the CNS.- Routes of Stem Cell Administration in the Adult Rodent.

From the reviews of the second edition:"Neural stem cells have been the focus of much research in an attempt to better understand the nature and behavior of these cells in vivo. … The purpose is to clarify research protocols in the investigation of neural stem cells. These worthy objectives are met. Neurologists, neurosurgeons, and neurosciences are the main audience. … This is a valuable acquisition for academic neuroscience, neurology, and neurosurgery libraries." (Celso Agner, Doody’s Review Service, June, 2008)"Neural Stem Cells: Methods and Protocols, Second Edition, provides a comprehensive overview on cutting-edge methods for scientists working on questions related to neural stem-cell biology. … The volume outlines methods and protocols that allow investigating mechanisms, which regulate self-renewal, cell survival and differentiation of NSCs. A better understanding of these processes is of great importance for future successful applications of NSCs in neurodegenerative diseases and injuries." (S. Atanasoski, Schweizer Archiv für Neurologie und Psychiatrie, April, 2009)