Neural Repair

Methods and Protocols

Inbunden, Engelska, 2023

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This detailed collection explores a diverse range of topics related to neural repair and functional recovery following ischemic stroke. In addition, several chapters focus on rodent stroke models and post-stroke functional evaluation, clinically-relevant therapeutic paradigms and co-morbidities, pharmacotherapy and methods of delivery.

Produktinformation

Utgivningsdatum2023-01-31
Mått178 x 254 x 33 mm
Vikt1 143 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor492
FörlagSpringer-Verlag New York Inc.
ISBN9781071629253

Tillhör följande kategorier

Rodent Stroke Models to Study Functional Recovery and Neural Repair.- Subcortical White Matter Stroke in the Mouse: Inducing Injury and Tracking Cellular Proliferation.- A Low Budget Photothrombotic Rodent Stroke Model.- Photothrombotic Model to Create an Infarct in the Hippocampus.- Bilateral Carotid Artery Stenosis and Cerebral Blood Flow Outcomes.- Internal Carotid Artery Stenosis: A Surgical Mouse Model to Study Moyamoya Syndrome.- Modeling Distal Middle Cerebral Artery Occlusion in Neonatal Rodents with Magnetic Nanoparticles or Magnetized Red Blood Cells.- In Vivo Imaging of the Structural Plasticity of Cortical Neurons after Stroke.- Measurement of Uninterrupted Cerebral Blood Flow by Laser Speckle Contrast Imaging (LSCI) during the Mouse Middle Cerebral Artery Occlusion Model by an Inverted LSCI Setup.- Multi-Exposure Speckle Imaging for Quantitative Evaluation of Cortical Blood Flow.- Wide-Field Optical Imaging in Mouse Models of Ischemic Stroke.- Post-Mortem Magnetic Resonance Imaging of Degenerating and Reorganizing White Matter in Post-Stroke Rodent Brain.- Quantitative Spatial Mapping of Axons across Cortical Regions to Assess Axonal Sprouting after Stroke.- Quantitative Evaluation of Cerebral Microhemorrhages in the Mouse Brain.- In Vivo Evaluation of BBB Integrity in the Post-Stroke Brain.- High Resolution RNA Sequencing from PFA-Fixed Microscopy Sections.- FACS to Identify Immune Subsets in Mouse Brain and Spleen.- A Guide on Analyzing Flow Cytometry Data Using Clustering Methods and Non-Linear Dimensionality Reduction (tSNE or UMAP).- Co-Culturing Immune Cells and Mouse-Derived Mixed Cortical Cultures with Oxygen-Glucose Deprivation to In Vitro Simulate Neuroinflammatory Interactions after Stroke.- Assessing Depression and Cognitive Impairment Following Stroke and Neurotrauma: Behavioral Methods for Quantifying Impairment and Functional Recovery.- Use of an Automated Mouse Touchscreen Platform for Quantification of Cognitive Deficits after Central Nervous System Injury.- Using Operant Reach Chambers to Assess Mouse Skilled Forelimb Use after Stroke.- The Finer Aspects of Grid-Walking and Cylinder Tests for Experimental Stroke Recovery Studies in Mice.- Performing Enriched Environment Studies to Improve Functional Recovery.- Clinically-Applicable Experimental Design and Considerations for Stroke Recovery Preclinical Studies.- Hydrogels and Nanoscaffolds for Long-Term Intraparenchymal Therapeutic Delivery after Stroke.- Reverse Translation to Develop Post-Stroke Therapeutic Interventions during Mechanical Thrombectomy: Lessons from the BACTRAC Trial.- Methods to Study Drug Uptake at the Blood-Brain Barrier Following Experimental Ischemic Stroke: In Vitro and In Vivo Approaches.- Gene Silencing in the Brain with siRNA to Promote Long-Term Post-Stroke Recovery.- Diabetic Rodent Models for Chronic Stroke Studies.- Use of Conventional Cigarette Smoking and E-Cigarette Vaping for Experimental Stroke Studies in Mice.- Middle Cerebral Artery Occlusion in Aged Animal Model.- Acute Ischemic Stroke by Middle Cerebral Artery Occlusion in Rat Models of Diabetes: Importance of Pre-Op and Post-Op Care, Severity of Hyperglycemia, and Sex.- The DOCA-Salt Model of Hypertension for Studies of Cerebrovascular Function, Stroke, and Brain Health.