Morpholino Oligomers

This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinosin uteroto developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Morpholino Oligomers: Methods and Protocolsaims to provide adiverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.