Molecular Microbial Ecology Manual

Inbunden, Engelska, 1995

AvA. D. L. Akkermans,etc.,A. D. Akkermans,Jan Dirk Van Elsas

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This manual provides general information about molecular techniques relevant to molecular ecological research. Attention is paid to the use of molecular methods for studying the fate of genetically modified and native microorganisms in the environment. Postgraduate students and research scientists in the plant and soils sciences and plant molecular biology should find this book of interest.

Produktinformation

Utgivningsdatum1995-10-31
FormatInbunden
SpråkEngelska
Antal sidor512
FörlagKluwer Academic Publishers
ISBN9780792336983

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Section 1: Isolation of Microbial Nucleic Acids. 1.1: Extraction of Microbial DNA from Aquatic Sources. 1.2: Extraction of Microbial RNA from Aquatic Sources. 1.3: Extraction of Microbial RNA from Bulk Soil. 1.4: Extraction of DNA from Phytosphere, Rhizosphere, Rhizoplane. 1.5: Extraction of RNA from Bulk Soil. 1.6: Nucleic Acid Extraction from Cultures. Section 2: Detection of Microbial Nucleic Acid Sequences. 2.2: Preparation of Radioactive Probes. 2.3: Preparation of Non-Radioactive Probes. 2.6: Detection of Microbial DNA Sequences by Colony Hybridization. 2.7: Detection and Quantification of Microbial Nucleic Acid Sequence Polymerase Chain Reaction (PCR). Section 3: Identification and Classification of Microbes Using DNA and RNA Sequences. 3.1: Partial and Complete 16S rDNA Sequences, Their Use in Generation of 16S rDNA Phylogenetic Trees and Their Implications in Molecular Ecological Studies. 3.3: Microbial identification and Design of Phylogenetic Trees Based on rRNA Sequences. Section 4: Detection, Identification and Classification of Microbes Using Other Methods. 4.1: Detection, Identification and Classification of Microbes Using Other Methods. Section 5: Detection of Gene Transfer in the Environment. 5.1: Gene Transfer by Transformation. 5.2: Gene Transfer by Conjugation. 5.3: Gene Transfer by Transduction. Section 6: Tracking of Specific Microbes in the Environment. 6.1: Marker Genes. 6.2: Designing Field and Microcosm Experiments with GEM's.