Mitosis and Meiosis Part A

Helder Maiato graduated in Biochemistry and holds a PhD in Biomedical Sciences from the University of Porto. He was a visiting PhD student at the University of Edinburgh (UK) and a Post-doctoral Research Affiliate at the New York State Department of Health (USA). At present, he is a Principal Investigator at the Institute for Research and Innovation in Health (i3S) and Invited Professor at the Faculty of Medicine of the University of Porto (Portugal). In 2015 he was honoured with the Young Investigator Award from the Louis-Jeantet Foundation and was recently elected EMBO Member. He served as National Counsellor for Science and Technology to the Portuguese Prime Minister and founded Yscience (www.yscience.pt), a pilot non-profit initiative that brings together scientists to promote science education in young children. His main research interest is the spatiotemporal control of chromosome segregation during cell division. Director, Department of Meiosis, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany

• PrefaceHelder Maiato and Melina Schuh1. Assays for the spindle assembly checkpoint in cell cultureChiara Marcozzi and Jonathon Pines2. Quantitative methods to measure aneuploidy and chromosomal instabilityKristina M. Godek and Duane A. Compton3. Dissecting the role of the tubulin code in mitosisLuísa T. Ferreira, Ana C. Figueiredo, Bernardo Orr, Danilo Lopes and Helder Maiato4. Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosisKara L. McKinley5. Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cellsBramwell G. Lambrus, Tyler C. Moyer and Andrew J. Holland6. Small molecule tools in mitosis researchFranziska Teusel, Lars Henschke and Thomas U. Mayer7. Optogenetic control of mitosis with photocaged chemical dimerizersHuaiying Zhang, David M. Chenoweth and Michael A. Lampson8. Measuring mitotic forcesAnna A. Ye and Thomas J. Maresca9. Employing the one-cell C. elegans embryo to study cell division processesNeil Hattersley, Pablo Lara-Gonzalez, Dhanya Cheerambathur, Sebastian Gomez, Taekyung Kim, Bram Prevo, Renat Khaliullin, Kian-Yong Lee, Midori Ota, Rebecca Green, Karen Oegema and Arshad Desai10. A cell-free system of Drosophila egg explants supporting native mitotic cyclesJorge de-Carvalho, Ojas Deshpande, Catarina Nabais and Ivo A. Telley11. Living Xenopus oocytes, eggs, and embryos as models for cell divisionAni Varjabedian, Angela Kita and William Bement12. Spreading of chromosomes from mouse oocytes and mammalian cultured cells for light microscopic analysisMariana C.C. Silva, Gordana Wutz, Kikuë Tachibana and Jan-Michael Peters13. In vitro reconstitution of lateral to end-on conversion of kinetochore–microtubule attachmentsManas Chakraborty, Ekaterina Tarasovetc and Ekaterina L. Grishchuk14. Isolation of mitotic chromosomes from vertebrate cells and characterization of their proteome by mass spectrometryItaru Samejima and William C. Earnshaw15. Purification of kinetochores from the budding yeast Saccharomyces cerevisiaeAmitabha Gupta, Rena K. Evans, Lori B. Koch, Aimee J. Littleton and Sue Biggins16. Characterization of DNA helicases and nucleases from meiotic extracts of S. cerevisiaeRokas Grigaitis, Aitor Susperregui, Philipp Wild and Joao Matos17. Single-nucleus Hi-C of mammalian oocytes and zygotesJohanna Gassler, Ilya M. Flyamer and Kikuë Tachibana18. Induction of fetal primary oocytes and the meiotic prophase from mouse pluripotent stem cellsHidetaka Miyauchi, Hiroshi Ohta and Mitinori Saitou19. Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryosGreg FitzHarris, John Carroll and Karl Swann20. Single cell genomics to study DNA and chromosome changes in human gametes and embryosRobert C. Blanshard, Chongyi Chen, X. Sunney Xie and Eva R. Hoffmann21. Cytoplasmic removal, enucleation, and cell fusion of mouse oocytesHirohisa Kyogoku, Shuhei Yoshida and Tomoya S. Kitajima