MHC Protocols

Inbunden, Engelska, 2002

AvStephen H. Powis,Robert W. Vaughan,Robert Vaughan

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A collection by leading experts detail of readily reproducible methods for identifying polymorphisms in the human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease (TAP1, TAP 2, C2, C4, and TNF- a). The authors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles.

Produktinformation

Utgivningsdatum2002-09-18
Mått155 x 235 x undefined mm
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor340
FörlagHumana Press Inc.
ISBN9780896035485

Tillhör följande kategorier

Onkologi inom Medicin
Biologi inom Naturvetenskap och teknik
Naturvetenskap:allmänt inom Naturvetenskap och teknik

Databases.- HLA Informatics.- Accessing HLA Sequencing Data Through the 6ace Database.- Polymorphism in Classical and Nonclassical Hla Genes.- HLA Typing by Restriction Fragment Length Polymorphism Analysis.- PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles.- PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR.- HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes.- PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles.- HLA Typing With Reference Strand-Mediated Conformation Analysis.- Sequencing Protocols for Detection of HLA Class I Polymorphism.- HLA-E and HLA-G Typing.- Typing Alleles of HLA-DM.- Polymorphism in Non-Hla Mhc Genes.- Typing Alleles of TAP1 and TAP2.- Determining Alleles of the C2 Gene by Southern Blotting.- Complement C4 Protein and DNA Typing Methods.- Typing of Tumor Necrosis Factor Alleles.- Molecular Typing of the MHC Class I Chain-Related Gene Locus.- Microsatellites.- HLA Microsatellite Analysis.