Methods in Tau Cell Biology

Dr. Feinstein earned his undergraduate degree in Biochemistry at the University of California, Berkeley and his Ph.D. from the Department of Biochemistry and Biophysics at the University of California School of Medicine, San Francisco. Subsequently, he was a postdoctoral fellow in the Department of Neurobiology at the Stanford University School of Medicine until 1986, at which time he joined the faculty at UCSB. He has served as a grant reviewer for the National Institutes of Health, the National Science Foundation and the Cancer Research Coordinating Committee of California. In 2011, he was appointed a National Academies Education Fellow in the Life Sciences by the National Academies of Science and previously has been awarded a UCSB Distinguished Teaching Award. Dr. Feinstein is presently Co-Director of the Neuroscience Research Institute. Dr. Lapointe has earned her undergraduate degree in Biology & Psychology at the University of Minnesota-Morris, and her Ph.D. in Neuroscience from Northwestern Univeristy. Subsequently, she was a postdoctoral fellow in Univeristy of California, Santa Barbara until 2013, and has continued to serve UCSB as an associate specialist. She is now an assistant project scientist at UCSB, and has participated in a number of research projects and publications, which her research is mainly focused on Tau proteins.

• 1. Recombinant tau expression and purificationStuart Feinstein2. In vitro MT dynamics and MT endsNichole LaPointe3. Methods related to investigating tau structure and MT bundlingCyrus R. Safinya4. Neurite outgrowth and retractionBrett Cook5. Methods related to studying tau fragmentationAdrianna Ferrerira6. Isoform-specific differences in tau in vitro aggregationTruman C. Gamblin7. Cosedimentation assays of microtubule bindingHolly Goodson8. Methods to study tau aggregationSongi Han9. Methods to study tau phosphorylationDiane Hanger10. Structure of tau oligomersNicholas M. Kanaan11. Use of mouse models for studies of pathological tau actionVirginia Lee and Sneha Narasimhan 12. Application of FRET and FCS to study tau structure-functionElizabeth Rhoades13. Use of the fly system to study tau actionMichelle Steinhilb14. Single molecule assays with motor proteinsChristopher Berger15. Tau clearance mechanisms in primary neurons with a focus on autophagyGail Johnson16. Structural studies of tauEckhard Mandelkow17. TIRF assays of tau effects on MT and actin cytoskeletons in vitroIsabelle Arnal18. TIRF assays of tau interactions with EB1Isabelle Arnal19. A novel approach to address tau biology, pathology, and propagation in vivo in mice using Adeno associated virusesSusanne Wegmann