Lipopolysaccharide Transport

Methods and Protocols

Inbunden, Engelska, 2022

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Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization.

Produktinformation

Utgivningsdatum2022-09-25
Mått178 x 254 x 22 mm
Vikt772 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor292
FörlagSpringer-Verlag New York Inc.
ISBN9781071625804

Tillhör följande kategorier

Cellbiologi inom Naturvetenskap och teknik

Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport.- Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa.- Analysing the Function of Essential Genes by Plasmid Shuffling.- Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia Coli.- Exploring The Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in silico and Biochemical Analyses.- Use of Site‐Directed Spin Labeling EPR Spectroscopy to Study Protein–LPS Interactions.- A Photo-Crosslinking Approach to Monitor Assembly of an LptD Intermediate with LptE in a Living Cell.- Mass Spectrometry Analysis of Dynamics and Interactions of the LPS translocon LptDE.- Affinity Purification and Co-Immunoprecipitation of Trans-envelope Protein Complexes in Gram-negative Bacteria.- A Bacterial Two-Hybrid System for in vivo assays of Protein-Protein Interactions and Drug Discovery.- Detecting Lipoproteins Sneaking out of the Lipopolysaccharide Leaflet.- Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry.- Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective.- Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA.- Protein Crystallization of Two Recombinant Lpt Proteins.- Metabolic Incorporation of Azido-Sugars into LPS to Enable Live Cell Fluorescence Imaging.- Use of Atomic Force Microscopy to Characterize LPS Perturbations.