Laboratory Rat Procedural Techniques

John Bogdanske After graduating from Augustana College, John started his research career in 1986 at the University of Wisconsin-Madison, Department of Nutritional Sciences working with dietary fiber and the nutritional value of common foods. In 1991 he moved to the School of Veterinary Medicine on the Madison campus to take the position of Lab Manager in the Comparative Orthopaedic Research Laboratory (CORL), Department of Medical Sciences. The CORL's mission was to investigate fundamental orthopaedic problems that affected either animals or humans in order to identify the mechanisms that cause disease or to develop treatments that would enhance the lives of individuals affected with these diseases. In 2004 he joined the Research Animal Resources Center (RARC) as a Training Coordinator. Along with Scott, Margaret and Beth, he helps develop, manage, and present a comprehensive training program to all UW-Madison research animal users and related staff. John is an active member of Laboratory Animal Welfare Training Exchange (LAWTE) and the American Association for Laboratory Animal Science (AALAS). John spends most of his free time with his family usually playing in the outdoors (on a bike).Scott Hubbard-Van Stelle Scott attended the University of Wisconsin in the pre-veterinary program before getting his degree as a certified veterinary technician from the Madison Area Technical College. He worked in a veterinary hospital for a small animal practitioner as a surgical nurse, followed by working for Raltech (now Covance) as a sample collection specialist. Scott started his career at the University of Wisconsin in 1980 when he took a position as a Veterinary Technician in the Veterinary Science Department (currently the department of Animal Health and Biomedical Sciences). After several years, he was promoted to Sr. Research Specialist. During his 19 years with the department, Scott set up the teachin

• DVD Contents: Rat Handling/Transfer. Rat Handling/Restraint. One-Handed Injection Technique. Intraperitoneal Injection (IP): One-Person. Intraperitoneal Injection (IP): Two-person. Intraperitoneal Injection (IP): One-Person Towel Method. Subcutaneous Injection (SQ): One-Person Towel Method. Pedal Vein Blood Draw. Saphenous Vein Blood Draw. Oral Gavage. Jugular Bleed (Two-Person). Tail Artery Blood Draw. Tail Vein Injection. Ear Notching. Ear Tagging. Rat Procedural Technique Manual & Handouts: Intraperitoneal Injection (IP). Subcutaneous Injection (SQ): Towel Method. Jugular Bleed. Oral Gavage. Pedal Vein Blood Draw. Saphenous Vein Blood Draw. Tail Artery Blood Draw. Tail Vein Injection. Ear Notch/Punch & Ear Tags. The Rat (Rattus norvegicus). Normative Data for the Laboratory Rat. Appendix.

The texts, Laboratory Rat and Laboratory Mouse Procedural Techniques Manual and DVD are short, easily read/watched manuals that review the very basic handling for the animal care technician through the clear use of discussion and accompanying photographs, enhanced with a clear, understandable DVD demonstrating each procedure discussed. Not only is the safety of the laboratory specimen of concern, but even more importantly, the safety of the human laboratory technicians and researchers. The reader is assumed to have fundamental laboratory rodent training in place. This is not meant as a primary training tool but to review and refresh existing understanding. Each of the texts is similar in organization and content which conveniently aids in navigation of topics. —ALN Magazine, 2012"While this handbook is not intended to present new information, it is a valuable desktop training resource for those who either want quick access to a biomethodology resource and those with responsibility for training and/ or supervising other individuals who need to use rats for research purposes and perform some or all of the techniques described. Additionally, the clear, step-by-step approach and simple instructions in both the printed manual and the DVD may be particularly helpful to those for whom English is a second language. In summary, this is a biomethodology training resource that many animal facilities using rats will find helpful to have on hand as an adjunct tool to existing training programs."—Patricia Foley, DVM, DACLAM, CPIA, in American Association for Laboratory Animal Science, May 2011, Vol 50, No 3, p. 412"Currently, there is a paucity of training material for experimental techniques in laboratory rodents, particularly audiovisual material. The manuals and accompanying DVDs will be of great interest to students, technicians, veterinarians, and investigators. Importantly, the straightforward approach taken in both the printed manual and DVD will be seen as an excellent tool for non-English speaking personnel."—Mark A. Suckow, DVM, Dipl. ACLAM, Director, Freimann Life Science Center, University of Notre Dame, & Past President of AALAS"Persons (students of animal technology) can watch these videos to 'get the picture' and then practice with an experienced individual. It is a good refinement step of the 3Rs…. I will be very excited to have these manuals to use as training items with my students and as reference resources in our animal facility."—Bruce W. Kennedy, MS, RLATG, CMAR, Compliance Associate, Research & Graduate Studies, California State Polytechnic University, Pomona, & Past President of AALAS