Del 539 - Methods in Enzymology

Laboratory Methods in Enzymology: Protein Part B

Inbunden, Engelska, 2014

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Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts.

Indispensable tool for the researcher
Carefully written and edited by experts to contain step-by-step protocols
In this volume we have brought together a number of core protocols concentrating on protein

Produktinformation

Utgivningsdatum2014-04-29
Mått152 x 229 x 16 mm
Vikt650 g
FormatInbunden
SpråkEngelska
SerieMethods in Enzymology
Antal sidor240
FörlagElsevier Science
MedarbetareLorsch,Jon
ISBN9780124201200

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Small-scale expression of proteins in E. coliProtein expression-YeastRecombinant protein expression in baculovirus-infected insect cellsSingle cell cloning of a stable mammalian cell lineIn vitro synthesis of proteins in bacterial extractsPreparation of a Saccharomyces cerevisiae cell-free extract for in vitro translationYeast two-hybrid screenUV cross-linking of interacting RNA and protein in cultured cellsAnalysis of RNA–protein interactions by cell mixingGeneral protein–protein cross-linkingChromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegansPAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteinsDetermining the RNA specificity and targets of RNA-binding proteins using a three-hybrid systemDissecting a known RNA–protein interaction using a yeast three-hybrid systemIdentifying proteins that bind a known RNA sequence using the yeast three-hybrid system