Laboratory Manual of Reproductive Biology and Assisted Reproductive Technologies

Jyoti Taneja is an Assistant Professor, with over 15 years of teaching experience in the Department of Zoology at Daulat Ram College, University of Delhi. Her research focuses on reproductive health, infectious disease epidemiology, and the application of machine learning in disease biology. She has a particular interest in polycystic ovary syndrome (PCOS), menstrual health, and reproductive tract infections, exploring how lifestyle factors influence women’s reproductive well-being.In addition to her work in reproductive health, Dr. Taneja applies AI-driven methods to improve diagnostic accuracy and support vaccine development. Her expertise in infectious disease epidemiology has been pivotal in understanding the dynamics of COVID-19, including disease severity, breakthrough infections, post-COVID sequelae, and vaccine hesitancy. Her research has contributed significantly to evidence-based public health policies and interventions, and is widely published in leading scientific journals.Priya Bhardwaj is a Scientist in the Department of Pharmacology at the All India Institute of Medical Sciences (AIIMS), New Delhi. She obtained her B.Sc. (Hons.) in Zoology, M.Sc. in Zoology, and Ph.D. from the University of Delhi, India. During her Master’s degree, she specialized in reproductive biology and subsequently received training in clinical research methods in public health.Her research paper got published in reputed National and International journals and has over five years of experience in conducting reproductive biology practicals for postgraduate students. Her research interests include proteomics-based cancer diagnostics, cardiovascular pharmacology, COVID-19, and reproductive health. Dr. Bhardwaj has also been recognized as a resource person in several workshops, refresher courses, and faculty development programs.

• Chapter 1: Set up and maintenance of Rodent Animal House facility; Chapter 2: Display of major endocrine glands and reproductive organ system in male and female rats; Chapter 3: Microscopic examinations of histological sections using illustrations/permanent slides/photomicrographs of human/rat; Chapter 4: Surgical techniques: Principles of surgery in endocrinology; Chapter 5: Dissect and display of the hypothalamic-hypophyseal organ system in rats; Chapter 6: To study the estrous cycle by observing the vaginal smear of adult female rats (Sprague-Dawley) under normal conditions; Chapter 7: Study of Exfoliative cytology of human cervix and vagina through micrographs; Chapter 8: Study of Sperm counting, Sperm Vitality and Sperm Motility in rats; Chapter 9: Study the Effect of Cryptorchidism on Sperm Count and Motility in Rats; Chapter 10: Study of Protein Profile of Rat Ovarian Extract by SDS-PAGE; Chapter 11: Separation of steroid hormones by Thin layer Chromatography and Paper chromatography; Chapter 12: To determine and quantify glycogen/ascorbic acid levels in rat uterine tissue; Chapter 13: To determine and quantify fructose in the dorsolateral prostate and coagulating glands of rat; Chapter 14: To Estimate Total Estrogens in Human Serum Samples Using Competitive ELISA technique; Chapter 15: To Understand the Methodology of a Pregnancy Test Kit; Chapter 16: Birth control methods: Study of modern contraceptive devices; Chapter 17: Retrieval of oocytes by induced superovulation in immature mice; Chapter 18: Study of causes of infertility in males and females and infertility investigation using secondary data, models and pictographs; Chapter 19: Setting up and Study of IVF Laboratory Culture Conditions; Chapter 20: Oocyte scoring and embryo quality grading in IVF for successful implantation; Chapter 21: Study of Assisted Reproductive Techniques; Chapter 22: Assisted Reproductive Technology: Ethical principles and laws in India; Chapter 23: Artificial Intelligence tools for Detection and Management of Sexually Transmitted Infections and Women's Reproductive Health: A Practical Approach for Undergraduate Students.