Integrated Stress Response

Methods and Protocols

Inbunden, Engelska, 2022

Av Daniel Matějů, Jeffrey A. Chao, Daniel Mateju, Daniel Matějů, Daniel Mat¿j¿

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This volume provides an up-to-date collection of protocols describing some of the key methods to investigate the integrated stress response (ISR), a vital evolutionarily conserved mechanism that enables eukaryotic cells to adapt to stress conditions and alter their gene expression programs.

Produktinformation

Utgivningsdatum2022-02-17
Mått178 x 254 x 29 mm
Vikt964 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor404
FörlagSpringer-Verlag New York Inc.
ISBN9781071619742

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An Overview of Methods for Detecting eIF2α Phosphorylation and the Integrated Stress Response.- CRISPR-Based Screening for Stress Response Factors in Mammalian Cells.- Multiplexed Analysis of Human uORF Regulatory Functions during the ISR Using PoLib-Seq.- Measuring Bulk Translation Activity in Single Mammalian Cells during the Integrated Stress Response.- Quantitative Translation Proteomics Using mePROD.- Quantifying the Binding of Fluorescently Labelled Guanine Nucleotides and Initiator tRNA to Eukaryotic Translation Initiation Factor 2.- Mammalian In Vitro Translation Systems.- Measuring Repeat-Associated Non-AUG (RAN) Translation.- Analysis of Ribosome Profiling Data.- Analysis of Translational Control in the Integrated Stress Response by Polysome Profiling.- High-Resolution Ribosome Profiling for Determining Ribosome Functional States during Translation Elongation.- Fluorescence Intensity-Based eIF2B’s Guanine Nucleotide-Exchange Factor Activity Assay.- Collective Learnings of Studies of Stress Granule Assembly and Composition.- Detecting Stress Granules in Drosophila Neurons.- Monitoring and Quantification of the Dynamics of Stress Granule Components in Living Cells by Fluorescence Decay after Photoactivation.- Probing Protein Solubility Patterns with Proteomics for Insight into Network Dynamics.- Analyzing the Composition and Organization of Ribonucleoprotein Complexes by APEX-Seq.- Differential Analysis of the Nuclear and the Cytoplasmic RNA Interactomes in Living Cells.- Identification of RNA-RBP Interactions in Subcellular Compartments by CLIP-Seq.- Monitoring Virus-Induced Stress Granule Dynamics Using Long-Term Live-Cell Imaging.- Single-Molecule Imaging of mRNA Interactions with Stress Granules.- Image-Based Screening for Stress Granule Regulators.- APEX Proximity Labeling of Stress Granule Proteins.