Insoluble Proteins

Methods and Protocols

Inbunden, Engelska, 2022

Av Elena Garcia Fruitós, Anna Arís Giralt, Elena Garcia Fruitos, Anna Aris Giralt

2 519 kr

Beställningsvara. Skickas inom 10-15 vardagar

Fri frakt för medlemmar vid köp för minst 249 kr.

Chapters describe recombinant protein production in different expression systems, prokaryotic and eukaryotic expression systems, purification protocols, characterization of insoluble proteins and a general overview of interesting applications of insoluble proteins.

Produktinformation

Utgivningsdatum2022-01-29
Mått178 x 254 x 35 mm
Vikt1 223 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor533
Upplaga2
FörlagSpringer-Verlag New York Inc.
ISBN9781071618585

Tillhör följande kategorier

Biovetenskap inom Naturvetenskap och teknik

General Introduction: Recombinant protein production and purification of insoluble proteins.- Overcoming the solubility problem in E. coli: available approaches for recombinant protein production.- A3D 2.0 update for the prediction and optimization of protein solubility.- Tailoring codon usage to the underlying biology for protein expression optimization.- Protein Expression Optimization Strategies in E. coli: A Tailored Approach in Strain Selection and Parallelizing Expression Conditions.- A novel approach for production of aggregation prone proteins using the spidroin-derived NT* tag.- Cleavable Self-Aggregating Tags (cSAT) for Therapeutic Peptide Expression and Purification.- Use Intein Cleavable Polyhydroxyalkanoate Synthase Fusions to Improve Protein Solubility.- Introduction of a hexalysine (6K) tag can protect from N-terminal cleavage and increase yield of recombinant proteins expressed in the periplasm of E. coli.- Two-tiered selection and screening strategy to increase functional enzyme production in Escherichia coli.- Method for production of cysteine-rich proteins in Lactococcus lactis expression system.- Heterologous protein production in Lactobacillus (plantarum) using pSIP vectors.- Soluble recombinant protein production in Pseudoaltermonas haloplanktis TAC125: the case study of the full-length human CDKL5 protein.- A protocol to enhance soluble protein expression in the cytoplasm of Bacillus subtilis.- Applications of cell-free synthesized membrane protein precipitates.- High cell-density expression system: yeast cells in a phalanx efficiently produce a certain range of “difficult-to-express” secretory recombinant proteins.- Insect Cells-Baculovirus system for the production of difficult to express proteins: from expression screening for soluble constructs to protein quality control.- Transient gene expression in human Expi293 cells.- Recombinant Expression of Complex Proteins in Human Cells Lines.- Optimizing chaperone removal strategy from over-expressed recombinant proteins: GNE, a case study.- Intrinsically disordered proteins (IDP): purification under denaturing conditions.- Solubilization and Refolding of Inclusion Body Proteins.- Non-denaturing solubilization of Inclusion Bodies from Lactic Acid Bacteria.- Purification of Inclusion Bodies Produced in Bacteria and Yeast.- Eukaryotic aggresomes: protocols and tips for their production, purification and handling.- Characterization of the conformational properties of soluble and insoluble proteins by Fourier transform infrared spectroscopy.- Characterizing Soluble Protein Aggregates using Native Mass Spectrometry Coupled with Temperature-control Electrospray Ionization and Size-exclusion Chromatography.- Quality control of proteins solubilized from inclusion bodies.- Methods for the Characterizationof Protein Aggregates.- Insoluble Protein Applications: The Use of Bacterial Inclusion Bodies as Biocatalysts.- Methods for Processing Protein Aggregates into Surfaces.