Industrial Bioseparations

Daniel Forciniti, Ph.D is a Professor at the Department of Chemical Engineering at the University of Missouri, Rolla. Dr. Forciniti joined the University of Missouri, Rolla after receiving his Ph.D. from North Carolina State University in 1991. Dr. Forciniti has published and made presentations extensively on chromatography, biomacromolecules, and biotechnology, including peer-reviewed articles, contributed chapters, and invited lectures.

• Preface. Chapter 1. Introduction to Bioseparations.a. Statement of the problem.b. Engineering Developments.c. Current approaches to bioseparations.Chapter 2. Thermodynamics.a. A brief introduction.b. Thermodynamics of open systems.c. Thermodynamic analysis of processes.Chapter 3. Principles of Mass Transfer.1. Diffusion.2. Fluxes.3. Law of conservation of mass.4. Examples.Chapter 4. General Properties of Bioproducts.1. Proteins.a. Structure.b. Stability (solubility and aggregation).c. Separation of complex protein mixtures by two-dimensional electrophoresis.2. Nucleic Acids.a. Structure.b. Properties.3. Laboratory experiments.Chapter 5. Cell disruption.1. General approach to cell disruption.a. Types of cells.b. Location of product.c. Physical Chemistry Properties of Cells (as they relate to disruption).2. Bead Mills.3. Homogenizers.4. Chemical Methods.5. Biochemical Methods.6. Laboratory experiments.Chapter 6. Separation of Solids.1. General Principles.a. Flocculation of proteins.b. Inclusion bodies.2. Filtration.a. Conventional Filtration.b. Ultrafiltration.3. Centrifugation.4. Sedimentation.5. Laboratory experiments.6. Liquid/liquid extraction.Chapter 7. Chromatography.1. General Principles.2. Gel permeation.3. Ion Exchange Chromatography.4. Hydrophobic Interactions Chromatography.5. Affinity Chromatography.6. Simulated Moving Bed Chromatography.7. Laboratory Experiments.Chapter 8. Protein Precipitation.1. Precipitation by salting-out.2. Isoelectric Precipitation.3. Precipitation by non-ionic polymers.4. Laboratory Experiments.Chapter 9. Polishing.1. Freeze drying.2. Crystallization.3. Product Formulation.4. Laboratory Experiments.Chapter 10. Separations in a Chip.1. Introduction to micro-devices.2. Fluidics.3. Micro-reactors, micro-mixers, and valves.4. Chromatography and electrophoresis in a chip.5. Micro-sensors.Chapter 11. Design of Separation cascades.1. General Principles.2. Examples.3. Laboratory Projects.Appendix. Experimental Design.1. Introduction to Experimental Design.2. Precision and Accuracy.3. Descriptive Statistics.4. Statistical Tests.5. Examples.Table of Contents.Chapter 1. Introduction to Bioseparations.a. Statement of the problem.b. Engineering Developments.c. Current approaches to bioseparations.Chapter 2. Thermodynamics.a. A brief introduction.b. Thermodynamics of open systems.c. Thermodynamic analysis of processes.Chapter 3. Principles of Mass Transfer.1. Diffusion.2. Fluxes.3. Law of conservation of mass.4. Examples.Chapter 4. General Properties of Bioproducts.1. Proteins.a. Structure.b. Stability (solubility and aggregation).c. Separation of complex protein mixtures by two-dimensional electrophoresis.2. Nucleic Acids.a. Structure.b. Properties.3. Laboratory experiments.Chapter 5. Cell disruption.1. General approach to cell disruption.a. Types of cells.b. Location of product.c. Physical Chemistry Properties of Cells (as they relate to disruption).2. Bead Mills.3. Homogenizers.4. Chemical Methods.5. Biochemical Methods.6. Laboratory experiments.Chapter 6. Separation of Solids.1. General Principles.a. Flocculation of proteins.b. Inclusion bodies.2. Filtration.a. Conventional Filtration.b. Ultrafiltration.3. Centrifugation.4. Sedimentation.5. Laboratory experiments.6. Liquid/liquid extraction.Chapter 7. Chromatography.1. General Principles.2. Gel permeation.3. Ion Exchange Chromatography.4. Hydrophobic Interactions Chromatography.5. Affinity Chromatography.6. Simulated Moving Bed Chromatography.7. Laboratory Experiments.Chapter 8. Protein Precipitation.1. Precipitation by salting-out.2. Isoelectric Precipitation.3. Precipitation by non-ionic polymers.4. Laboratory Experiments.Chapter 9. Polishing.1. Freeze drying.2. Crystallization.3. Product Formulation.4. Laboratory Experiments.Chapter 10. Separations in a Chip.1. Introduction to micro-devices.2. Fluidics.3. Micro-reactors, micro-mixers, and valves.4. Chromatography and electrophoresis in a chip.5. Micro-sensors.Chapter 11. Design of Separation cascades.1. General Principles.2. Examples.3. Laboratory Projects.Appendix. Experimental Design.1. Introduction to Experimental Design.2. Precision and Accuracy.3. Descriptive Statistics.4. Statistical Tests.5. Examples.Index

"This volume will serve as an invaluable introduction and guide to those embarking on careers in the discipline of bioseparations. Forciniti deserves our thanks for promoting knowledge of this important endeavor." (Pharmaceutical Technology, November 2, 2008)