Immunoassays in Coagulation Testing

Häftad, Engelska, 2012

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Rapid progress in analytical methods, within the past few decades, has led to the widespread applications of newer immunological and radioimmunoassay techniques to the diagnosis and treatment of hemorrhagic and thrombotic disorders.

Produktinformation

Utgivningsdatum2012-05-18
Mått155 x 235 x 11 mm
Vikt300 g
FormatHäftad
SpråkEngelska
Antal sidor173
FörlagSpringer-Verlag New York Inc.
ISBN9781461572275

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Onkologi inom Medicin
Medicin: allmänt inom Medicin
Biokemi inom Naturvetenskap och teknik
Cellbiologi inom Naturvetenskap och teknik
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1. Basic Concepts in Coagulation.- Hemostasis.- Vasoconstriction.- Prostaglandins and Platelet Aggregation.- Coagulation Cascade.- Fibrinolysis.- Serine Protease Inhibitors.- 2. Pathophysiology of Coagulation Disorders.- Hemophilia-A.- Hemophilia-B (Christmas Disease).- von Willebrand’s Disease (vWd).- Disseminated Intravascular Coagulation (DIC).- Platelet Function Defects.- Hypercoagulability.- Laboratory Testing.- Conclusions.- 3. Basic Concepts in Clinical Immunology.- Antigens (Immunogens).- Antigenic Determinant Sites.- Antibodies.- Antigen Binding Sites.- Structure.- Antibody Specificity.- Monoclonal Antibodies.- 4. Review of Immunologic Techniques.- Radial Immunodiffusion (RID).- Electroimmunodiffusion (EID).- Crossed Immunoelectrophoresis (CIEP).- Turbidimetric Immunoassay (TIA).- Nephelometric Immunoassay (NIA).- Radioimmunoassay (RIA).- Immunoradiometric Assay (IRMA).- Fluoroimmunoassay (FIA).- Enzyme-linked Immunosorbent Assay (ELISA).- Thin Layer Immunoassay (TLIA).- Particle Counting Immunoassay (PACIA).- Latex Agglutination Test.- Clinical Considerations.- Selected Bibliography.- 5. Procedure for Immunoassays.- Proteins of the Coagulation System.- Proteins of the Fibrinolytic System.- Serine Protease Inhibitors.- Platelet Specific Proteins.- 6. Immunotechniques in Coagulation Research.- Double Immunodiffusion.- Crossed Immunoelectrophoresis.- Antibody Neutralization Assay.- Radioimmunoassay.- Immunoadsorption.- Conclusions.- 7. Quality Control.- Immunologic Reagents.- Standard Preparation.- Internal Standard.- Units of Concentration.- Procedure.- Quality Control Charts.- 8. Future Outlook.- Conclusions.- 9. Appendix A. Techniques in Antibody Production.- Antigen Purification.- Isolation of AT-III.- Isolation of Plasminogen.- Protein Determination.-Immunization.- Preparation of Antiserum.- FITC-labeling of Antibodies.- 10. Appendix B. Blood Collecting Techniques.- Double Syringe Method.- Vacutainer Method.- 11. Appendix C. Buffers and Stains.- Phosphate Buffer.- Veronal, Barbital, or Barbitone Buffer.- Veronal Acetate Buffer pH 5.6–8.3.- Phosphate Buffered Saline (PBS).- 0.05 M Tris Buffer pH 7.2–8.6.- Stains.- Coomassie Blue.- Amido Black.- Azocarmine B.- Nigrosin.- Sudan Black B.- Destaining Solution.- Appendix D. Normal Ranges of Coagulation Proteins in Plasma.- Appendix E. Manufacturers of Immunologic Reagents and Equipment.- North American Region.- European Region.- Appendix F. Glossary of Terms.- References.